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AB236959

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody

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Rabbit Polyclonal Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CHRNB1 aa 1-250.

View Alternative Names

ACHRB, CHRNB, CHRNB1, Acetylcholine receptor subunit beta

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)

Paraffin-embedded human colon cancer tissue stained for Nicotinic Acetylcholine Receptor beta/CHRNB1 using ab236959 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)

PC-3 (Human prostate adenocarcinoma cell line) cells stained for Nicotinic Acetylcholine Receptor beta/CHRNB1 (green) using ab236959 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)

Paraffin-embedded human liver cancer tissue stained for Nicotinic Acetylcholine Receptor beta/CHRNB1 using ab236959 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)
  • WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (AB236959)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody (ab236959) at 1/500 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate

Lane 3:

HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human CHRNB1 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P11230

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor beta known commonly as CHRNB1 or CHRNB forms part of the nicotinic acetylcholine receptors (nAChRs). This receptor is a transmembrane protein channel that participates in the transmission of signals through the synaptic cleft by responding to the neurotransmitter acetylcholine. CHRNB1 has a molecular mass of approximately 58 kDa. Researchers find this receptor member expressed in neuromuscular junctions and in several areas of the central nervous system including the brain and spinal cord providing essential roles in synaptic transmission.
Biological function summary

CHRNB1 functions as a component of the pentameric ion channel complex that forms upon binding with other subunits like the alpha subunit of nAChR. When acetylcholine binds to this channel it permits the flow of cations such as sodium and calcium into cells which leads to depolarization and subsequent neurotransmission. This process is fundamental for various neurological functions including muscle contraction and cognitive processes. The receptor is critical for maintaining the efficiency of synaptic connectivity and signaling.

Pathways

CHRNB1 plays a role in neural pathways such as the cholinergic and dopaminergic pathways influencing neurotransmission and behavior. In the cholinergic pathway nAChRs including CHRNB1 modulate the release of other neurotransmitters and are integral to processes like learning and memory. Additionally associations with proteins like CHRNA1 and CHRNA4 illustrate the receptor's involvement in ensuring proper synaptic signaling and response to neurotransmitters across neural circuits.

Dysfunction or mutations in CHRNB1 correlate with conditions like congenital myasthenic syndromes and may contribute to cognitive disorders such as Alzheimer's disease. In congenital myasthenic syndromes impaired signaling influences muscle function highlighting its neuromuscular importance. Researchers also associate CHRNB1 interactions with amyloid precursor protein (APP) in Alzheimer's disease highlighting the receptor's involvement in neurodegenerative processes and its potential as a therapeutic target for modulating synaptic dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
See full target information CHRNB1
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