AB284747

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

ACHRB, CHRNB, CHRNB1, Acetylcholine receptor subunit beta

3 Images

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

This data was developed using ab76159, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] (<a href='/en-us/products/primary-antibodies/nicotinic-acetylcholine-receptor-beta-chrnb1-antibody-ep2067y-ab76159'>ab76159</a>) at 1/1000 dilution

All lanes:

Mouse Brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

This data was developed using ab76159, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Rat Brain lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Unknown

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

This data was developed using ab76159, the same antibody clone in a different buffer formulation.

All lanes:

Human brain lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 57 kDa

Observed band size: 57 kDa

false

Unconjugated

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2067Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab284747 is the carrier-free version of ab76159

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor beta known commonly as CHRNB1 or CHRNB forms part of the nicotinic acetylcholine receptors (nAChRs). This receptor is a transmembrane protein channel that participates in the transmission of signals through the synaptic cleft by responding to the neurotransmitter acetylcholine. CHRNB1 has a molecular mass of approximately 58 kDa. Researchers find this receptor member expressed in neuromuscular junctions and in several areas of the central nervous system including the brain and spinal cord providing essential roles in synaptic transmission.

Biological function summary

CHRNB1 functions as a component of the pentameric ion channel complex that forms upon binding with other subunits like the alpha subunit of nAChR. When acetylcholine binds to this channel it permits the flow of cations such as sodium and calcium into cells which leads to depolarization and subsequent neurotransmission. This process is fundamental for various neurological functions including muscle contraction and cognitive processes. The receptor is critical for maintaining the efficiency of synaptic connectivity and signaling.

Pathways

CHRNB1 plays a role in neural pathways such as the cholinergic and dopaminergic pathways influencing neurotransmission and behavior. In the cholinergic pathway nAChRs including CHRNB1 modulate the release of other neurotransmitters and are integral to processes like learning and memory. Additionally associations with proteins like CHRNA1 and CHRNA4 illustrate the receptor's involvement in ensuring proper synaptic signaling and response to neurotransmitters across neural circuits.

Dysfunction or mutations in CHRNB1 correlate with conditions like congenital myasthenic syndromes and may contribute to cognitive disorders such as Alzheimer's disease. In congenital myasthenic syndromes impaired signaling influences muscle function highlighting its neuromuscular importance. Researchers also associate CHRNB1 interactions with amyloid precursor protein (APP) in Alzheimer's disease highlighting the receptor's involvement in neurodegenerative processes and its potential as a therapeutic target for modulating synaptic dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

See full target information CHRNB1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

Western blot - Anti-Nicotinic Acetylcholine Receptor beta/CHRNB1 antibody [EP2067Y] - BSA and Azide free (AB284747)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Product promise