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AB233831

Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6]

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(3 Publications)

Mouse Monoclonal Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody. Suitable for WB and reacts with Rat, Recombinant full length protein - Human, Transfected cell lysate - Human, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CHRNE aa 1-250.

View Alternative Names

ACHRE, CHRNE, Acetylcholine receptor subunit epsilon

3 Images
Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)
  • WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (ab233831) at 1/500 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 2:

C6 (Rat glial tumor cell line) cell lysate

Lane 3:

SK-N-SH (human neuroblastoma cell line) cell lysate

Predicted band size: 54 kDa

false

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)
  • WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)

Expected MW is 50.1 kDa.

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (ab233831) at 1/500 dilution

All lanes:

Recombinant human Nicotinic Acetylcholine Receptor epsilon/CHRNE protein

Predicted band size: 54 kDa

false

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)
  • WB

Supplier Data

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (AB233831)

All lanes:

Western blot - Anti-Nicotinic Acetylcholine Receptor epsilon/CHRNE antibody [4E10F6] (ab233831) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Nicotinic Acetylcholine Receptor epsilon/CHRNE (aa: 21-239)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 54 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4E10F6

Isotype

IgG1

Carrier free

No

Reacts with

Rat, Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human CHRNE aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q04844

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Nicotinic Acetylcholine Receptor epsilon also known as CHRNE is a subunit of the nicotinic acetylcholine receptor found in the neuromuscular junction. This receptor plays a critical role in signal transduction across synaptic clefts by mediating the effect of the neurotransmitter acetylcholine. In molecular terms CHRNE functions as part of the pentameric ligand-gated ion channel. It exhibits involvement in the conversion of chemical signals into electrical ones by facilitating the flow of cations like sodium and calcium. The estimated mass of this target is approximately 57 kDa and it is expressed primarily in skeletal muscle tissue.
Biological function summary

The CHRNE subunit fits into the nicotinic acetylcholine receptor complex which involves other subunits like alpha beta delta and gamma. This receptor complex agonistically responds to the binding of acetylcholine released from motor neurons which ultimately leads to muscle contraction. The epsilon subunit replaces the gamma subunit during the development from fetal to adult muscle. This adjustment increases the affinity of the receptor to acetylcholine ensuring an enhanced efficiency in synaptic transmission.

Pathways

The CHRNE subunit plays a part in significant biological pathways including the neuromuscular synaptic transmission and the downstream process of muscle contraction. It associates with the agrin-LRP4-MuSK pathway which is essential for the formation and maintenance of the neuromuscular junction. Additionally it interacts with rapsyn a protein important for clustering and anchoring these receptors at the neuromuscular junction. Through these important pathways CHRNE ensures effective neuromuscular communication.

CHRNE relates to congenital myasthenic syndromes which feature muscle weakness due to impaired neuromuscular transmission. Mutations in the CHRNE gene can disrupt receptor assembly or function leading to these syndromes. It also associates with myasthenia gravis an autoimmune disorder where the immune system attacks components of the neuromuscular junction often targeting proteins like acetylcholine receptors. Understanding the role of CHRNE and its interactions with these proteins provides insight into therapeutic approaches for these neuromuscular diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
See full target information CHRNE
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cells 13: PubMed39273021

2024

Cell-Sonar, a Novel Method for Intracellular Tracking of Secretory Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Thomas Seeger,Franz Worek,Simone Rothmiller

Archives of medical science : AMS 20:1631-1654 PubMed39649262

2023

Oxymatrine inhibits the development of radioresistance in NSCLC cells by reversing EMT through the DcR3/AKT/GSK-3β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jianming Tang,Yu Cao,Hong Zhang,Rui Wang

The Journal of surgical research 241:308-316 PubMed31055156

2019

Alleviating Sepsis-Induced Neuromuscular Dysfunction Linked With Acetylcholine Receptors by Agrin.

Applications

Unspecified application

Species

Unspecified reactive species

Bixiao Lv,Su Min,Fei Xie,Jun Yang,Jingyuan Chen
View all publications
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