AB131279

Anti-Nidogen 2/NID-2 antibody [EPR8697]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Recombinant Monoclonal Nidogen 2/NID-2 antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Nidogen-2, NID-2, Osteonidogen, NID2

2 Images

Unknown

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

All lanes:

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (ab131279) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

BxPC-3 lysate at 10 µg

Lane 3:

Human fetal heart lysate at 10 µg

Lane 4:

Human fetal lung lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 151 kDa

false

OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8697

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'Nidogen 2' also called 'NID-2' or '"nidogen protein'" or '"nid-2"' is a glycoprotein found in basement membranes. It has a molecular mass around 150 kDa and expresses across various tissues particularly in the vascular structures and certain epithelial tissues. This protein has sulfated and carbohydrate-rich regions making it important in forming stable extracellular matrix architecture.

Biological function summary

'nidogen 2' plays roles in maintaining the structural integrity of basement membranes. It interacts with other matrix components like laminin and collagen IV forming part of complexes that ensure the basal membrane's maintenance and stability. 'NID-2' provides structural support and helps secure cellular environments which is important for tissue repair and morphogenesis.

Pathways

'nidogen 2' contributes to the extracellular matrix remodeling and cell signaling pathways. It participates in the integrin signaling pathway where it interacts with proteins such as 'integrin' and 'perlecan'. These interactions help mediate cellular adhesion and signal transduction impacting cellular behavior like proliferation differentiation and migration processes.

Abnormalities in 'nidogen 2' relate to certain cancers and kidney disorders. In cancer disrupted interactions with 'laminin' may promote tumor cell invasion and metastasis. In kidney disorders such as nephrotic syndrome altered 'nidogen 2' function relates to basement membrane defects affecting kidney filtration function. Understanding its role could lead to insights into therapies for these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cell adhesion glycoprotein which is widely distributed in basement membranes. Binds to collagens I and IV, to perlecan and to laminin 1. Does not bind fibulins. It probably has a role in cell-extracellular matrix interactions.

See full target information Nidogen-2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 9:159 PubMed37173310

2023

N-Methyladenosine modification of mRNA regulates neuronal gene expression and oxygen glucose deprivation/reoxygenation induction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangyang Qi,Chi Zhang,Huan Jian,Mengfan Hou,Yongfu Lou,Yi Kang,Wei Wang,Yigang Lv,Shenghui Shang,Chaoyu Wang,Xueying Li,Shiqing Feng,Hengxing Zhou

Nature communications 9:5150 PubMed30514914

2018

Spatially and functionally distinct subclasses of breast cancer-associated fibroblasts revealed by single cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Bartoschek,Nikolay Oskolkov,Matteo Bocci,John Lövrot,Christer Larsson,Mikael Sommarin,Chris D Madsen,David Lindgren,Gyula Pekar,Göran Karlsson,Markus Ringnér,Jonas Bergh,Åsa Björklund,Kristian Pietras

Bioscience, biotechnology, and biochemistry 80:1344-55 PubMed26966997

2016

Effects of C-xylopyranoside derivative on epithelial regeneration in an in vitro 3D oral mucosa model.

Applications

Unspecified application

Species

Unspecified reactive species

Atsushi Uenoyama,Ikuko Kakizaki,Aki Shiomi,Naoaki Saito,Yuko Hara,Taro Saito,Hisashi Ohnuki,Hiroko Kato,Ritsuo Takagi,Takeyasu Maeda,Kenji Izumi

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nidogen 2/NID-2 antibody [EPR8697]

Western blot - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

OI-RD Scanning - Anti-Nidogen 2/NID-2 antibody [EPR8697] (AB131279)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise