JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB121000

Anti-Niemann Pick C1 Like 1/NPC1L1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Goat Polyclonal NPC1-like intracellular cholesterol transporter 1 precursor antibody. Cited in 2 publications.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

WB

applications

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Niemann Pick C1 Like 1 (NPC1L1) also known simply as NPC1L1 is a protein that plays a critical role in the intestinal absorption of dietary cholesterol. NPC1L1 is a multi-pass membrane protein located in the brush border of small intestine enterocytes and also present in the liver. It consists of approximately 1334 amino acids giving it a mass of around 148 kDa. This protein serves as a conduit for cholesterol as it moves across the cell membrane taking part in the cholesterol uptake mechanism.
Biological function summary

NPC1L1 is essential for maintaining cholesterol homeostasis within the body. It is not part of a larger protein complex but works closely with other proteins to facilitate lipid incorporation into chylomicrons. Inhibitors of NPC1L1 such as ezetimibe effectively reduce blood cholesterol levels by blocking this absorption route. The presence of NPC1L1 on the apical surface of enterocytes emphasizes its function in the body's cholesterol metabolism impacting systemic lipid levels.

Pathways

NPC1L1 is involved with cholesterol absorption pathways an important component of lipid homeostasis. This pathway involves other important proteins such as the Niemann-Pick C1 protein (NPC1) and ATP-binding cassette transporters like ABCG5 and ABCG8. These proteins along with NPC1L1 coordinate the regulation of cholesterol levels by balancing absorption and excretion aiding in maintaining overall homeostasis in the body.

NPC1L1's role in cholesterol regulation links it directly to atherosclerosis and hypercholesterolemia. Elevated levels of cholesterol can lead to plaque formation in arteries contributing to coronary artery disease. Ezetimibe a drug targeting NPC1L1 demonstrates this connection as it helps lower cholesterol levels by inhibiting NPC1L1 function. Moreover interactions with other proteins like APOB (Apolipoprotein B) and LDL receptor exemplify the broader implication of NPC1L1 in lipid metabolism disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information NPC1-like intracellular cholesterol transporter 1 precursor
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 11:602115 PubMed33363473

2020

- and -Palmitoleic Acid Isomers Regulate Cholesterol Metabolism in Different Ways.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Wen Huang,Bi-Hong Hong,Kai-Kai Bai,Ran Tan,Ting Yang,Ji-Peng Sun,Rui-Zao Yi,Hao Wu

Scientific reports 5:10512 PubMed26015368

2015

Microbiota prevents cholesterol loss from the body by regulating host gene expression in mice.

Applications

WB

Species

Mouse

Chun-Yan Zhong,Wei-Wei Sun,Yinyan Ma,Hongling Zhu,Pan Yang,Hong Wei,Ben-Hua Zeng,Qian Zhang,Yu Liu,Wen-Xia Li,Yixin Chen,Liqing Yu,Zhi-Yuan Song
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB124801

Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]

RabMAb|Recombinant

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)

  • 5
  • 1
Primary Antibodies

AB201773

HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]

RabMAb|Recombinant

Western blot - HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB201773)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB134113

Anti-Niemann Pick C1 antibody [EPR5209]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-Niemann Pick C1 antibody [EPR5209] (AB134113)

  • 5
  • 2
Primary Antibodies

AB30682

Anti-SREBP2 antibody

Western blot - Anti-SREBP2 antibody (AB30682)

  • 4
  • 11
Primary Antibodies

AB66217

Anti-ABCA1 antibody [HJ1]

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ABCA1 antibody [HJ1] (AB66217)

  • 3
  • 3
Primary Antibodies

AB52818

Anti-LDL Receptor antibody [EP1553Y]

RabMAb|KO Validated|Recombinant

Western blot - Anti-LDL Receptor antibody [EP1553Y] (AB52818)

  • 4
  • 16

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com