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AB124801

Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]

5

(1 Review)

|

(10 Publications)

Rabbit Recombinant Monoclonal Niemann Pick C1 Like 1/NPC1L1 antibody. Suitable for WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

NPC1-like intracellular cholesterol transporter 1, NPC1L1, Niemann-Pick C1-like protein 1

3 Images
Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)
  • WB

Unknown

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)

All lanes:

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution

Lane 1:

Human fetal liver lysates at 10 µg

Lane 2:

HeLa lysates at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 149 kDa

false

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)
  • WB

Lab

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/10000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 102 kDa,149 kDa,20 kDa,24 kDa,29 kDa,30 kDa,31 kDa,33 kDa,51 kDa,55 kDa,73 kDa,82 kDa

Observed band size: 102 kDa,149 kDa,20 kDa,24 kDa,29 kDa,42 kDa,51 kDa,65 kDa,73 kDa,75 kDa,78 kDa

false

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)
  • WB

Lab

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (AB124801)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] (ab124801) at 1/1000 dilution

All lanes:

HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 149 kDa,45 kDa

Observed band size: 149 kDa,45 kDa

false

  • Carrier free

    Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717] - BSA and Azide free

  • HRP

    HRP Anti-Niemann Pick C1 Like 1/NPC1L1 antibody [EPR5717]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5717

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Niemann Pick C1 Like 1 (NPC1L1) also known simply as NPC1L1 is a protein that plays a critical role in the intestinal absorption of dietary cholesterol. NPC1L1 is a multi-pass membrane protein located in the brush border of small intestine enterocytes and also present in the liver. It consists of approximately 1334 amino acids giving it a mass of around 148 kDa. This protein serves as a conduit for cholesterol as it moves across the cell membrane taking part in the cholesterol uptake mechanism.
Biological function summary

NPC1L1 is essential for maintaining cholesterol homeostasis within the body. It is not part of a larger protein complex but works closely with other proteins to facilitate lipid incorporation into chylomicrons. Inhibitors of NPC1L1 such as ezetimibe effectively reduce blood cholesterol levels by blocking this absorption route. The presence of NPC1L1 on the apical surface of enterocytes emphasizes its function in the body's cholesterol metabolism impacting systemic lipid levels.

Pathways

NPC1L1 is involved with cholesterol absorption pathways an important component of lipid homeostasis. This pathway involves other important proteins such as the Niemann-Pick C1 protein (NPC1) and ATP-binding cassette transporters like ABCG5 and ABCG8. These proteins along with NPC1L1 coordinate the regulation of cholesterol levels by balancing absorption and excretion aiding in maintaining overall homeostasis in the body.

NPC1L1's role in cholesterol regulation links it directly to atherosclerosis and hypercholesterolemia. Elevated levels of cholesterol can lead to plaque formation in arteries contributing to coronary artery disease. Ezetimibe a drug targeting NPC1L1 demonstrates this connection as it helps lower cholesterol levels by inhibiting NPC1L1 function. Moreover interactions with other proteins like APOB (Apolipoprotein B) and LDL receptor exemplify the broader implication of NPC1L1 in lipid metabolism disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a major role in cholesterol homeostasis (PubMed : 22095670). Critical for the uptake of cholesterol across the plasma membrane of the intestinal enterocyte (PubMed : 22095670). Involved in plant sterol absorption, it transports sitosterol, although at lower rates than cholesterol (By similarity). Is the direct molecular target of ezetimibe, a drug that inhibits cholesterol absorption and is approved for the treatment of hypercholesterolemia (PubMed : 15928087). May have a function in the transport of multiple lipids and their homeostasis, thereby influencing lipid metabolism regulation (PubMed : 15671032). May be involved in caveolin trafficking from the plasma membrane (By similarity). In addition, acts as a negative regulator of NPC2 and down-regulates its expression and secretion by inhibiting its maturation and accelerating its degradation (PubMed : 22095670).
See full target information NPC1-like intracellular cholesterol transporter 1
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature metabolism 7:102-119 PubMed39779889

2025

Intestinal TM6SF2 protects against metabolic dysfunction-associated steatohepatitis through the gut-liver axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Zhang,Harry Cheuk-Hay Lau,Suki Ha,Chuanfa Liu,Cong Liang,Hye Won Lee,Queena Wing-Yin Ng,Yi Zhao,Fenfen Ji,Yunfei Zhou,Yasi Pan,Yang Song,Yating Zhang,Jennie Ching Yin Lo,Alvin Ho Kwan Cheung,Jianfeng Wu,Xiaoxing Li,Hongzhi Xu,Chi Chun Wong,Vincent Wai-Sun Wong,Jun Yu

Neural regeneration research 19:642-649 PubMed37721296

2023

Treatment with β-sitosterol ameliorates the effects of cerebral ischemia/reperfusion injury by suppressing cholesterol overload, endoplasmic reticulum stress, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiuling Tang,Tao Yan,Saiying Wang,Qingqing Liu,Qi Yang,Yongqiang Zhang,Yujiao Li,Yumei Wu,Shuibing Liu,Yulong Ma,Le Yang

Foods (Basel, Switzerland) 10: PubMed34945647

2021

Interaction between Flavonoids and Carotenoids on Ameliorating Oxidative Stress and Cellular Uptake in Different Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Chen,Zeyuan Deng,Liufeng Zheng,Bing Zhang,Ting Luo,Hongyan Li

Foods (Basel, Switzerland) 10: PubMed34829135

2021

The Effects of Anthocyanin-Rich Bilberry Extract on Transintestinal Cholesterol Excretion.

Applications

Unspecified application

Species

Unspecified reactive species

Jimin Hong,Minji Kim,Bohkyung Kim

Proceedings of the National Academy of Sciences of 117:32105-32113 PubMed33239446

2020

Cholesterol 25-hydroxylase suppresses SARS-CoV-2 replication by blocking membrane fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Ruochen Zang,James Brett Case,Eylan Yutuc,Xiucui Ma,Sheng Shen,Maria Florencia Gomez Castro,Zhuoming Liu,Qiru Zeng,Haiyan Zhao,Juhee Son,Paul W Rothlauf,Alex J B Kreutzberger,Gaopeng Hou,Hu Zhang,Sayantan Bose,Xin Wang,Michael D Vahey,Kartik Mani,William J Griffiths,Tom Kirchhausen,Daved H Fremont,Haitao Guo,Abhinav Diwan,Yuqin Wang,Michael S Diamond,Sean P J Whelan,Siyuan Ding

Molecular medicine reports 20:3224-3232 PubMed31432115

2019

Hepatic NPC1L1 overexpression attenuates alcoholic autophagy in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Youlin Wang,Pan Yang,Bo Zhang,Yao Ding,Shun Lei,Yuning Hou,Xiaoqing Guan,Qingwang Li

Lipids 53:217-233 PubMed29569242

2018

Egg-Yolk Sphingomyelin and Phosphatidylcholine Attenuate Cholesterol Absorption in Caco-2 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Guoxun Chen,Meihu Ma,Ning Qiu,Lingjiao Zhu,Jing Li

Lipids in health and disease 17:32 PubMed29463265

2018

Fatty acids modulate the expression levels of key proteins for cholesterol absorption in Caco-2 monolayer.

Applications

Unspecified application

Species

Unspecified reactive species

Fang Yang,Guoxun Chen,Meihu Ma,Ning Qiu,Lingjiao Zhu,Jing Li

Journal of lipid research 55:681-97 PubMed24493833

2014

A lincRNA-DYNLRB2-2/GPR119/GLP-1R/ABCA1-dependent signal transduction pathway is essential for the regulation of cholesterol homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Wei Hu,Jun-Yao Yang,Xin Ma,Zhi-Ping Chen,Ya-Rong Hu,Jia-Yi Zhao,Shu-Fen Li,Yu-Rong Qiu,Jing-Bo Lu,Yan-Chao Wang,Ji-Juan Gao,Yan-Hua Sha,Lei Zheng,Qian Wang

BMC gastroenterology 13:30 PubMed23406058

2013

Role of the ABCG8 19H risk allele in cholesterol absorption and gallstone disease.

Applications

WB

Species

Unspecified reactive species

Olga Renner,Dieter Lütjohann,Dominique Richter,André Strohmeyer,Silke Schimmel,Oliver Müller,Eduard F Stange,Simone Harsch
View all publications
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Product promise

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