Anti-Niemann Pick C2 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (AB186829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification : 400x.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Niemann Pick C2 antibody (AB186829)

Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186829. Blue DAPI for nuclear staining.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (AB186829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification : 400x.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (AB186829)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification : 200x.

• WB

Supplier Data

Western blot - Anti-Niemann Pick C2 antibody (AB186829)

All lanes:

Western blot - Anti-Niemann Pick C2 antibody (ab186829) at 1/500 dilution

Lane 1:

293T cell extract

Lane 2:

M21 cell extract

Lane 3:

Mouse lung cell extract

Lane 4:

Mouse spleen cell extract

Lane 5:

Mouse liver cell extract

Predicted band size: 17 kDa

false

• WB

CiteAb

Western blot - Anti-Niemann Pick C2 antibody (AB186829)

Niemann Pick C2 western blot using anti-Niemann Pick C2 antibody ab186829. Publication image and figure legend from Yagi, H., Nishigori, M., et al., 2020, Sci Rep, PubMed 32286426.

ab186829 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186829 please see the product overview.

Expression and localization of NPC2, IGFBP7, and THBS1 in aortic tissues. (A) Protein levels of THBS1, IGFBP7, and NPC2 in aortic tissues of NVDCs and those of three different stages (Stages A, B, and C) of TAAA patients were determined by western blot analysis. ACTB was used as an internal control. Arrows indicate the predicted positions of THBS1 (129 kDa), IGFBP7 (29 kDa), NPC2 (17 kDa), and ACTB (42 kDa), respectively. Whole gel images are presented in Supplementary Fig. 2. (B) Immunohistochemical analyses of NPC2, IGFBP7, and THBS1 (first, second, and third row, respectively); Staining data for normal aortas of NVDCs are shown in a, e, and i of the left column, while those of TAAAs are shown in b-d of the first, f-h of the second, and j-l of the third rows. Arrows in d indicate NPC2-positive foam cells. Arrows in f, g, and h indicate IGFBP7-positive foam cells, endothelial cells, and smooth muscle cells, respectively. Arrowheads in g indicate IGFBP7-positive smooth muscle cells. The asterisks in e and g indicate the lumenal region. NVDC, non-vascular disease control; TAAA, thoracic atherosclerotic aortic aneurysm.

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