Rabbit Polyclonal Niemann Pick C2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPC2.
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Publications
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- Scientific reports 10:64292020Discovery of novel biomarkers for atherosclerotic aortic aneurysm through proteomics-based assessment of disease progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHiroaki Yagi et. al.PubMed 32286426
- Scientific reports 7:31452017Imipramine Inhibits Chikungunya Virus Replication in Human Skin Fibroblasts through Interference with Intracellular Cholesterol Trafficking.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSineewanlaya Wichit,Rodolphe Hamel,Eric Bernard,Loïc Talignani,Fodé Diop,Pauline Ferraris,Florian Liegeois,Peeraya Ekchariyawat,Natthanej Luplertlop,Pornapat Surasombatpattana,Frédéric Thomas,Andres Merits,Valérie Choumet,Pierre Roques,Hans Yssel,Laurence Briant,Dorothée MisséPubMed 28600536
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NPC2. The exact immunogen used to generate this antibody is proprietary information. Database link P61916
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Species Mouse | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:11125141, PubMed:15937921, PubMed:17018531, PubMed:18772377, PubMed:29580834). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).
Alternative names
HE1, NPC2, NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein, He1
Recommended products
Rabbit Polyclonal Niemann Pick C2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NPC2.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NPC2. The exact immunogen used to generate this antibody is proprietary information. Database link P61916
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Niemann Pick C2 protein (NPC2) is a small intracellular cholesterol transport protein weighing approximately 16 kDa. Alternately referred to as "NPC2 protein" or "NPC 293" it binds with lipids and is predominantly expressed in the lysosome. This protein plays a critical role in the intracellular lipid transport process. NPC2 forms part of a family of proteins tasked with moving cholesterol and other related lipids within lysosomal compartments.
- Biological function summary
NPC2 protein facilitates the transfer of cholesterol from lysosomes to other parts of the cell. It operates in tandem with its partner protein Niemann Pick C1 (NPC1) to mediate the egress of cholesterol. The NPC2 protein docks to NPC1 within the lysosomal membrane forming an essential complex in lipid transport. This function is pivotal for the maintenance of cellular cholesterol homeostasis influencing various cellular and systemic functions linked to cholesterol metabolism.
- Pathways
NPC2 protein participates prominently in the cholesterol efflux pathways. It associates with the NPC1 protein to regulate the movement of cholesterol from the lysosomal membrane to cytoplasmic areas where it can be utilized or stored. This pathway links NPC2 to significant biological processes like the homeostasis of lipoprotein-derived cholesterol influencing the SREBP pathway which governs lipid synthesis and uptake. Such interactions highlight NPC2's involvement in regulating cellular lipid balance.
- Associated diseases and disorders
NPC2 protein dysfunction notably links to Niemann-Pick disease type C a lipid storage disorder. Mutations in the genes encoding NPC2 or NPC1 disrupt cholesterol transportation leading to abnormal lipid accumulation and progressive neurodegeneration. Additionally this protein's dysregulation is indirectly connected to atherosclerosis as such disruptions can result in altered cellular cholesterol management highlighting its interaction with proteins involved in cardiovascular diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Niemann Pick C2 antibody (ab186829)
All lanes: Western blot - Anti-Niemann Pick C2 antibody (ab186829) at 1/500 dilution
Lane 1: 293T cell extract
Lane 2: M21 cell extract
Lane 3: Mouse lung cell extract
Lane 4: Mouse spleen cell extract
Lane 5: Mouse liver cell extract
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab186829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab186829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 200x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Niemann Pick C2 antibody (ab186829)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Niemann Pick C2 with ab186829 at 1/200. Magnification: 400x.
Immunocytochemistry/ Immunofluorescence - Anti-Niemann Pick C2 antibody (ab186829)
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186829. Blue DAPI for nuclear staining.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Niemann Pick C2 antibody (ab186829)
Niemann Pick C2 western blot using anti-Niemann Pick C2 antibody ab186829. Publication image and figure legend from Yagi, H., Nishigori, M., et al., 2020, Sci Rep, PubMed 32286426.
ab186829 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186829 please see the product overview.
Expression and localization of NPC2, IGFBP7, and THBS1 in aortic tissues. (A) Protein levels of THBS1, IGFBP7, and NPC2 in aortic tissues of NVDCs and those of three different stages (Stages A, B, and C) of TAAA patients were determined by western blot analysis. ACTB was used as an internal control. Arrows indicate the predicted positions of THBS1 (129 kDa), IGFBP7 (29 kDa), NPC2 (17 kDa), and ACTB (42 kDa), respectively. Whole gel images are presented in Supplementary Fig. 2. (B) Immunohistochemical analyses of NPC2, IGFBP7, and THBS1 (first, second, and third row, respectively); Staining data for normal aortas of NVDCs are shown in a, e, and i of the left column, while those of TAAAs are shown in b-d of the first, f-h of the second, and j-l of the third rows. Arrows in d indicate NPC2-positive foam cells. Arrows in f, g, and h indicate IGFBP7-positive foam cells, endothelial cells, and smooth muscle cells, respectively. Arrowheads in g indicate IGFBP7-positive smooth muscle cells. The asterisks in e and g indicate the lumenal region. NVDC, non-vascular disease control; TAAA, thoracic atherosclerotic aortic aneurysm.
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