Rabbit Recombinant Monoclonal Niemann Pick C2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (AB244952), expandable thumbnail](https://content.abcam.com/products/images/niemann-pick-c2-antibody-epr19993-63-bsa-and-azide-free-detector-ab244952--sandwich-elisa-img52767.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (AB244952)")
![Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (AB244952), expandable thumbnail](https://content.abcam.com/products/images/niemann-pick-c2-antibody-epr19993-63-bsa-and-azide-free-detector-ab244952--sandwich-elisa-img410011.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (AB244952)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Intracellular cholesterol transporter which acts in concert with NPC1 and plays an important role in the egress of cholesterol from the lysosomal compartment (PubMed:17018531, PubMed:11125141, PubMed:18772377, PubMed:29580834, PubMed:15937921). Unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes is transferred by NPC2 to the cholesterol-binding pocket in the N-terminal domain of NPC1 (PubMed:17018531, PubMed:18772377, PubMed:27238017). May bind and mobilize cholesterol that is associated with membranes (PubMed:18823126). NPC2 binds cholesterol with a 1:1 stoichiometry (PubMed:17018531). Can bind a variety of sterols, including lathosterol, desmosterol and the plant sterols stigmasterol and beta-sitosterol (PubMed:17018531). The secreted form of NCP2 regulates biliary cholesterol secretion via stimulation of ABCG5/ABCG8-mediated cholesterol transport (By similarity).
Alternative names
NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein, He1, HE1, NPC2
Recommended products
Rabbit Recombinant Monoclonal Niemann Pick C2 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
NPC intracellular cholesterol transporter 2, Epididymal secretory protein E1, Human epididymis-specific protein 1, Niemann-Pick disease type C2 protein, He1, HE1, NPC2
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR19993-63
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952) is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify human Niemann Pick C2.
The recommended pair for sandwich ELISA is:
Capture: Anti-Niemann Pick C2 antibody [EPR19993-4] - BSA and Azide free (Capture) ab309200, human Niemann Pick C2 Capture Antibody (unconjugated)
Detector: ab244952, human Niemann Pick C2 Detector Antibody (unconjugated)
The reference range value is 7.8-500 pg/ml
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- Activity summary
The Niemann Pick C2 protein (NPC2) is a small intracellular cholesterol transport protein weighing approximately 16 kDa. Alternately referred to as "NPC2 protein" or "NPC 293" it binds with lipids and is predominantly expressed in the lysosome. This protein plays a critical role in the intracellular lipid transport process. NPC2 forms part of a family of proteins tasked with moving cholesterol and other related lipids within lysosomal compartments.
- Biological function summary
NPC2 protein facilitates the transfer of cholesterol from lysosomes to other parts of the cell. It operates in tandem with its partner protein Niemann Pick C1 (NPC1) to mediate the egress of cholesterol. The NPC2 protein docks to NPC1 within the lysosomal membrane forming an essential complex in lipid transport. This function is pivotal for the maintenance of cellular cholesterol homeostasis influencing various cellular and systemic functions linked to cholesterol metabolism.
- Pathways
NPC2 protein participates prominently in the cholesterol efflux pathways. It associates with the NPC1 protein to regulate the movement of cholesterol from the lysosomal membrane to cytoplasmic areas where it can be utilized or stored. This pathway links NPC2 to significant biological processes like the homeostasis of lipoprotein-derived cholesterol influencing the SREBP pathway which governs lipid synthesis and uptake. Such interactions highlight NPC2's involvement in regulating cellular lipid balance.
- Associated diseases and disorders
NPC2 protein dysfunction notably links to Niemann-Pick disease type C a lipid storage disorder. Mutations in the genes encoding NPC2 or NPC1 disrupt cholesterol transportation leading to abnormal lipid accumulation and progressive neurodegeneration. Additionally this protein's dysregulation is indirectly connected to atherosclerosis as such disruptions can result in altered cellular cholesterol management highlighting its interaction with proteins involved in cardiovascular diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952), expandable thumbnail](https://content.abcam.com/products/images/niemann-pick-c2-antibody-epr19993-63-bsa-and-azide-free-detector-ab244952--sandwich-elisa-img52767.jpg "Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952)")
Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human Niemann Pick C2 ELISA Kit ab227894).
![Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952), expandable thumbnail](https://content.abcam.com/products/images/niemann-pick-c2-antibody-epr19993-63-bsa-and-azide-free-detector-ab244952--sandwich-elisa-img410011.jpg "Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952)")
Sandwich ELISA - Anti-Niemann Pick C2 antibody [EPR19993-63] - BSA and Azide free (Detector) (ab244952)
Sandwich ELISA of Human Niemann Pick C2 Antibody Pair - BSA and Azide free ab309250 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.
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