AB231181

Anti-Ninein/NIN antibody

Name: Anti-Ninein/NIN antibody (ab231181) | Abcam
Brand: Abcam Limited
SKU: AB231181

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(3 Publications)

Rabbit Polyclonal Ninein antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 3 publications.

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Paraffin-embedded rat heart tissue stained for Ninein/NIN using ab231181 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Formalin-fixed, paraffin-embedded rat stomach tissue stained for Ninein/NIN using ab231181 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Paraffin-embedded rat cerebrum tissue stained for Ninein/NIN using ab231181 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-Ninein/NIN antibody (AB231181)

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Western blot - Anti-Ninein/NIN antibody (ab231181) at 2 µg/mL

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K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate

Predicted band size: 243 kDa

false

Supplier Data

Western blot - Anti-Ninein/NIN antibody (AB231181)

All lanes:

Western blot - Anti-Ninein/NIN antibody (ab231181) at 2 µg/mL

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Recombinant rat Ninein/NIN protein

Predicted band size: 243 kDa

false

Supplier Data

Western blot - Anti-Ninein/NIN antibody (AB231181)

All lanes:

Western blot - Anti-Ninein/NIN antibody (ab231181) at 1 µg/mL

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Rat testis lysate

Predicted band size: 243 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-20 µg/mL", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231181 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ninein also known as NIN or Antiabrin is a centrosomal protein important for microtubule anchoring. It has a molecular mass of approximately 230 kDa. This protein is expressed in several tissues with heightened levels in nerve and epithelial cells. Ninein localizes primarily at the centrosome and is an important centrosome marker aiding in the attachment of microtubules at the centrosome during cell division.

Biological function summary

Ninein is critical for proper cell cycle progression and cellular organization. It acts as a scaffolding protein playing a significant role in centrosome cohesion and microtubule anchoring by interacting with other centrosome proteins. Ninein is also a part of the pericentriolar matrix a component of the centrosome necessary for microtubule nucleation and stabilization ensuring the correct formation and orientation of the mitotic spindle.

Pathways

Ninein plays a significant role in centrosome-related signaling pathways including microtubule organization and maintenance. It interacts with several proteins such as rootletin influencing centrosome cohesion and dynamics. Ninein is also implicated in the cell cycle regulation pathway where it works alongside pericentrin to maintain centrosome integrity and support chromosomal segregation.

Ninein has been linked to ciliopathies and some neurological disorders. In ciliopathies Ninein dysfunction can lead to improper cilia formation and function highlighting its role in cilia biogenesis. Moreover disruptions in Ninein expression or function can contribute to brain development disorders as it is involved in neuronal differentiation. Proteins such as centrin are often studied alongside Ninein to better understand its involvement in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ninein

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35119360

2022

α-/γ-Taxilin are required for centriolar subdistal appendage assembly and microtubule organization.

Applications

Unspecified application

Species

Unspecified reactive species

Dandan Ma,Fulin Wang,Rongyi Wang,Yingchun Hu,Zhiquan Chen,Ning Huang,Yonglu Tian,Yuqing Xia,Junlin Teng,Jianguo Chen

Journal of dental sciences 16:1146-1153 PubMed34484582

2021

MiR-874-3p inhibits osteogenic differentiation of human periodontal ligament fibroblasts through regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shengzhang Song,Zhuanjun Yan,Wangxi Wu

eLife 9: PubMed32965215

2020

Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2.

Applications

Unspecified application

Species

Unspecified reactive species

Yosuke Tona,Doris K Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ninein/NIN antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ninein/NIN antibody (AB231181)

Western blot - Anti-Ninein/NIN antibody (AB231181)

Western blot - Anti-Ninein/NIN antibody (AB231181)

Western blot - Anti-Ninein/NIN antibody (AB231181)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise