Rabbit Polyclonal Ninein/NIN antibody. Centrosome marker. Suitable for ICC/IF and reacts with Human samples. Cited in 31 publications.
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- eLife 9:2020Live imaging of hair bundle polarity acquisition demonstrates a critical timeline for transcription factor Emx2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Tona et. al.PubMed 32965215
- PLoS biology 18:e30006792020Acute inhibition of centriolar satellite function and positioning reveals their functions at the primary cilium.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÖzge Z Aydin et. al.PubMed 32555591
- PLoS genetics 14:e10076642018Disrupted structure and aberrant function of CHIP mediates the loss of motor and cognitive function in preclinical models of SCAR16.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-He Shi,Carrie Rubel,Sarah E Soss,Rebekah Sanchez-Hodge,Shuo Zhang,Sabrina C Madrigal,Saranya Ravi,Holly McDonough,Richard C Page,Walter J Chazin,Cam Patterson,Cheng-Yuan Mao,Monte S Willis,Hai-Yang Luo,Yu-Sheng Li,Donte A Stevens,Mi-Bo Tang,Pan Du,Yao-He Wang,Zheng-Wei Hu,Yu-Ming Xu,Jonathan C SchislerPubMed 30222779
- The Journal of cell biology 217:1205-12152018Centrobin controls primary ciliogenesis in vertebrates.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYetunde Adesanya Ogungbenro et. al.PubMed 29440264
- Scientific reports 7:14742017Spindle pole cohesion requires glycosylation-mediated localization of NuMA.
- Molecular biology of the cell 28:736-7452017Centriole splitting caused by loss of the centrosomal linker protein C-NAP1 reduces centriolar satellite density and impedes centrosome amplification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnne-Marie Flanagan et. al.PubMed 28100636
- Molecular biology of the cell 28:120-1272016A role for Gle1, a regulator of DEAD-box RNA helicases, at centrosomes and basal bodies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-En Jao,Abdalla Akef,Susan R WentePubMed 28035044
- Reproduction in domestic animals = Zuchthygiene 52 Suppl 2:193-1962016Deciphering the mechanisms involving cenexin, ninein and centriolin in sperm maturation during epididymal transit in the domestic cat.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT Rowlison,M A Ottinger,P ComizzoliPubMed 27807895
- Scientific reports 6:26580201614-3-3γ Prevents Centrosome Amplification and Neoplastic Progression.Applications:ICC/IFReactive species:Unspecified reactive speciesAmitabha Mukhopadhyay et. al.PubMed 27253419
- Journal of cell science 129:1769-742016CEP164-null cells generated by genome editing show a ciliation defect with intact DNA repair capacity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOwen M Daly et. al.PubMed 26966185
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
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Target data
Function
Centrosomal protein required in the positioning and anchorage of the microtubule minus-end in epithelial cells (PubMed:15190203, PubMed:23386061). May also act as a centrosome maturation factor (PubMed:11956314). May play a role in microtubule nucleation, by recruiting the gamma-tubulin ring complex to the centrosome (PubMed:15190203). Overexpression does not perturb nucleation or elongation of microtubules but suppresses release of microtubules (PubMed:15190203). Required for centriole organization and microtubule anchoring at the mother centriole (PubMed:23386061).
Alternative names
KIAA1565, NIN, Ninein, hNinein, Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein
Recommended products
Rabbit Polyclonal Ninein/NIN antibody. Centrosome marker. Suitable for ICC/IF and reacts with Human samples. Cited in 31 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
The centrosomal protein ninein has been identified as a microtubule minus end capping and centriole anchoring protein, but its true physiological function remains to be determined. At mitosis, ninein has been observed in association with the mitotic spindle as well as at the centrosomes.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Ninein also known as NIN or Antiabrin is a centrosomal protein important for microtubule anchoring. It has a molecular mass of approximately 230 kDa. This protein is expressed in several tissues with heightened levels in nerve and epithelial cells. Ninein localizes primarily at the centrosome and is an important centrosome marker aiding in the attachment of microtubules at the centrosome during cell division.
- Biological function summary
Ninein is critical for proper cell cycle progression and cellular organization. It acts as a scaffolding protein playing a significant role in centrosome cohesion and microtubule anchoring by interacting with other centrosome proteins. Ninein is also a part of the pericentriolar matrix a component of the centrosome necessary for microtubule nucleation and stabilization ensuring the correct formation and orientation of the mitotic spindle.
- Pathways
Ninein plays a significant role in centrosome-related signaling pathways including microtubule organization and maintenance. It interacts with several proteins such as rootletin influencing centrosome cohesion and dynamics. Ninein is also implicated in the cell cycle regulation pathway where it works alongside pericentrin to maintain centrosome integrity and support chromosomal segregation.
- Associated diseases and disorders
Ninein has been linked to ciliopathies and some neurological disorders. In ciliopathies Ninein dysfunction can lead to improper cilia formation and function highlighting its role in cilia biogenesis. Moreover disruptions in Ninein expression or function can contribute to brain development disorders as it is involved in neuronal differentiation. Proteins such as centrin are often studied alongside Ninein to better understand its involvement in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)
ab4447 staining Ninein in HeLa cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab4447 (shown in green) at 0.1μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (shown in red), Mouse monoclonal to alpha Tubulin, at 1μg/ml. The secondary antibodies used were Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L), and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Alexa Fluor® 594 Goat Anti-Mouse IgG (H&L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Gordon Chan, University of AlbertaImmunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (ab4447)
IF using ab4447 to detect Ninein/NIN in MCF7 cells (human).
The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein/NIN.
ab4447 was used at 1/1000.
Ninein/NIN shows nuclear and centrosome staining, which is expected from the literature.
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