Anti-Ninein/NIN antibody - Centrosome Marker

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (AB4447)

IF using ab4447 to detect Ninein/NIN in MCF7 cells (human).

The left hand panels are DAPI stained nuclei. The right hand panels are the same nuclei stained for Ninein/NIN.

ab4447 was used at 1/1000.

Ninein/NIN shows nuclear and centrosome staining, which is expected from the literature.

Gordon Chan, University of Alberta

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Ninein/NIN antibody - Centrosome Marker (AB4447)

ab4447 staining Ninein in HeLa cells. The cells were fixed with 4% PFA (10 mins), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab4447 (shown in green) at 0.1μg/ml and ab7291 (shown in red), Mouse monoclonal to alpha Tubulin, at 1μg/ml. The secondary antibodies used were ab150081, Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L), and ab150120, Alexa Fluor® 594 Goat Anti-Mouse IgG (H&L) used at a 1/1000 dilution for 1h at room temperature. Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.