AB172627

Anti-Ninjurin2 antibody [EPR12038]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal Ninjurin2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications.

View Alternative Names

Ninjurin-2, Nerve injury-induced protein 2, NINJ2

2 Images

Supplier Data

Immunoprecipitation - Anti-Ninjurin2 antibody [EPR12038] (AB172627)

Western blot analysis on immunoprecipitation pellet from (1) fetal lung lysate or (2) 1XPBS (negative control) using ab172627, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

Lane 1 Primary : ab172627, 1/1000 dilution
Sample : Fetal lung lysate

Lane 2 Primary : ab172627, 1/1000 dilution
Sample : 1XPBS (negative control)

All lanes:

Immunoprecipitation - Anti-Ninjurin2 antibody [EPR12038] (ab172627) at 1/1000 dilution

Lane 1:

Fetal lung lysate

Lane 2:

1X PBS (negative control)

Predicted band size: 16 kDa

false

Supplier Data

Western blot - Anti-Ninjurin2 antibody [EPR12038] (AB172627)

All lanes:

Western blot - Anti-Ninjurin2 antibody [EPR12038] (ab172627) at 1/1000 dilution

Lane 1:

Fetal lung lysate at 10 µg

Lane 2:

Human spleen lysate at 10 µg

Predicted band size: 16 kDa

false

Carrier free

Anti-Ninjurin2 antibody [EPR12038] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12038

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ninjurin2 also known as NINJ2 is a member of the Ninjurin protein family. It weighs approximately 16 kDa and mainly expresses in nerve tissues and some peripheral organs. Ninjurin2 is a transmembrane protein that plays a role in cell adhesion and migration. It interacts with the extracellular matrix and supports the interaction between cells. The protein’s structural features include hydrophobic domains that aid in this binding.

Biological function summary

Ninjurin2 contributes to the repair and regeneration of nerve tissues. It actively engages in cellular communication promoting neurogenesis and synapse formation in the nervous system. Ninjurin2 is not known as part of a larger protein complex but it functions alongside molecules involved in cellular regeneration. Research suggests a significant involvement in the modulation of immune responses interfacing with immune cells during injury response.

Pathways

Ninjurin2 functions within important neural and immune signaling routes. It participates in the neuroplasticity pathway which is critical for healing neural injuries and supports the immune surveillance system by communicating with cytokines. The protein works closely with other related proteins like Ninjurin1 which shares similar cellular roles as well as integrins which help mediate its cellular adhesion functions.

Ninjurin2 has been linked to conditions related to nerve damage and inflammation such as neurodegenerative disorders and chronic inflammatory diseases. In cases of neurodegenerative disorders abnormal Ninjurin2 function may affect neuron durability and regeneration. Connections with inflammatory diseases arise due to its role in immune response modulation. Ninjurin2’s interaction with other proteins in these contexts notably MyD88 affects cellular responses to injury and subsequent recovery processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Its role in unclear (PubMed : 33472215, PubMed : 38614101, PubMed : 39667936). In contrast to NINJ1 paralog, does not mediate plasma membrane rupture (cytolysis) downstream of necroptotic and pyroptotic programmed cell death (PubMed : 33472215, PubMed : 38614101, PubMed : 39667936). While it is able to oligomerize and form filaments, filaments are curved toward the intracellular space, preventing circularization to mediate plasma membrane rupture (PubMed : 39667936). May act as a homophilic transmembrane adhesion molecule involved in nerve regeneration (PubMed : 10627596). Promotes axonal growth (PubMed : 10627596).

See full target information NINJ2

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:2224-2235.e16 PubMed38614101

2024

NINJ1 mediates plasma membrane rupture by cutting and releasing membrane disks.

Applications

Unspecified application

Species

Unspecified reactive species

Liron David,Jazlyn P Borges,L Robert Hollingsworth,Allen Volchuk,Isabelle Jansen,Evelyn Garlick,Benjamin E Steinberg,Hao Wu

Aging 11:11136-11147 PubMed31794427

2019

Ninjurin2 overexpression promotes glioma cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Na Zhou,Ping Li,Shang Cai,Gang Li,Fang Liu

Aging 11:8526-8541 PubMed31597121

2019

Ninjurin 2 overexpression promotes human colorectal cancer cell growth and .

Applications

Unspecified application

Species

Unspecified reactive species

Gang Li,Li-Na Zhou,Han Yang,Xixi He,Yuxia Duan,Fang Wu

Biochemical and biophysical research communication 505:1180-1188 PubMed30318119

2018

Targeting ninjurin 2 by miR-764 regulates hydrogen peroxide (HO)-induced neuronal cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Ding Jing,Li Yinzhu,Pang Jinjing,Liang Lishuang,Zhang Guozhuan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ninjurin2 antibody [EPR12038]

Immunoprecipitation - Anti-Ninjurin2 antibody [EPR12038] (AB172627)

Western blot - Anti-Ninjurin2 antibody [EPR12038] (AB172627)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise