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AB63557

Anti-NIPA (phospho S354) antibody

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(2 Publications)

Rabbit Polyclonal NIPA phospho S354 antibody. Suitable for WB and reacts with African green monkey samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ZC3HC1 pS354.

View Alternative Names

NIPA, HSPC216, ZC3HC1, Zinc finger C3HC-type protein 1, Nuclear-interacting partner of ALK, Nuclear-interacting partner of anaplastic lymphoma kinase, hNIPA

1 Images
Western blot - Anti-NIPA (phospho S354) antibody (AB63557)
  • WB

Unknown

Western blot - Anti-NIPA (phospho S354) antibody (AB63557)

All lanes:

Western blot - Anti-NIPA (phospho S354) antibody (ab63557) at 1/500 dilution

Lane 1:

extracts from COS7 cells, treatedwith HU (2nM, 24hours) at 30 µg

Lane 2:

extracts from COS7 cells, treatedwith HU (2nM, 24hours) at 30 µg with immunizing phosphopeptide

Predicted band size: 55 kDa

Observed band size: 30 kDa,55 kDa,75 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

African green monkey

Applications

WB

applications

Immunogen

Synthetic Peptide within Human ZC3HC1 pS354. The exact immunogen used to generate this antibody is proprietary information.

Q86WB0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for proper positioning of a substantial amount of TPR at the nuclear basket (NB) through interaction with TPR.
See full target information ZC3HC1 pS354
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 13:13-20 PubMed26572258

2015

Methylene blue exerts a neuroprotective effect against traumatic brain injury by promoting autophagy and inhibiting microglial activation.

Applications

WB

Species

Mouse

Mingfei Zhao,Feng Liang,Hangdi Xu,Wei Yan,Jianmin Zhang

The Journal of biological chemistry 287:37997-8005 PubMed22955283

2012

Extracellular signal-regulated kinase 2 (ERK2) mediates phosphorylation and inactivation of nuclear interaction partner of anaplastic lymphoma kinase (NIPA) at G2/M.

Applications

WB

Species

Unspecified reactive species

Anna Lena Illert,Michael Zech,Cathrin Moll,Corinna Albers,Stefanie Kreutmair,Christian Peschel,Florian Bassermann,Justus Duyster
View all publications
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Product promise

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