JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB322205

Anti-Nipah virus Glycoprotein G antibody [EPR29883-707]

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Glycoprotein G antibody. Suitable for WB, I-ELISA and reacts with Transfected cell lysate - Nipah virus, Recombinant fragment - Nipah virus samples.

View Alternative Names

Glycoprotein G, G

2 Images
Indirect ELISA - Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] (AB322205)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] (AB322205)

Indirect ELISA analysis of ab322205 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution.

Antigen : Nipah virus Glycoprotein G.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] (AB322205)
  • WB

Supplier Data

Western blot - Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] (AB322205)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with Hendra virus Glycoprotein G.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] (ab322205) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag, whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a Nipah virus Glycoprotein G expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a Hendra virus Glycoprotein G expression vector containing a myc-his-tag, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 75 kDa,36 kDa

false

Exposure time: 3s

  • Carrier free

    Anti-Nipah virus Glycoprotein G antibody [EPR29883-707] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29883-707

Isotype

IgG

Carrier free

No

Reacts with

Nipah virus

Applications

I-ELISA, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Nipah virus": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Nipah virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "1000 ng/mL", "IELISA-species-notes": "<p></p>" }, "Transfected cell lysate - Nipah virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nipah virus Glycoprotein G also known as NiV-G serves mechanically as an essential viral attachment protein. This glycoprotein with a mass of approximately 70 kDa is expressed on the surface of the Nipah virus envelope. It facilitates viral entry into host cells by binding to ephrin-B2 and ephrin-B3 receptors. Its activity is necessary for initiating infection in host species including humans and animals.
Biological function summary

Glycoproteins like Nipah virus Glycoprotein G play a significant role in viral life cycles. NiV-G exists as a part of a complex and works in conjunction with other viral proteins especially the fusion protein F to mediate membrane fusion. By binding selectively to host cell receptors it determines the host range and tissue tropism of the virus. The interaction with host receptors also impacts the viral spread within the host organism.

Pathways

Several viral entry mechanisms involve Nipah virus Glycoprotein G. The fusion process involving this glycoprotein and the fusion protein (F protein) is a part of the cell entry pathway. This pathway is critical for the virus to access intracellular machinery. Additionally NiV-G is connected with signaling pathways through its interaction with ephrin receptors contributing to viral pathogenesis and immune evasion.

Nipah virus Glycoprotein G is significantly associated with Nipah virus infection leading to severe encephalitis and respiratory illness. This protein through its interaction with ephrin-B2 and ephrin-B3 contributes to the virus's ability to cause widespread cell damage in the brain and respiratory tissues. Moreover the glycoprotein F works in concert with NiV-G to facilitate transmission and heightened virulence of the infection highlighting the critical role of these proteins in disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Interacts with host ephrinB2/EFNB2 or ephrin B3/EFNB3 to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-mediated endocytosis.
See full target information Glycoprotein G
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB43812

Anti-Respiratory Syncytial Virus antibody [2F7]

Immunocytochemistry/ Immunofluorescence - Anti-Respiratory Syncytial Virus antibody [2F7] (AB43812)

  • 5
  • 1
Primary Antibodies

AB214337

Anti-Flavivirus NS1 antibody [D/2/D6/B7]

Immunocytochemistry/ Immunofluorescence - Anti-Flavivirus NS1 antibody [D/2/D6/B7] (AB214337)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB32352

Anti-HIV1 p24 antibody [P131]

RabMAb|Recombinant

Western blot - Anti-HIV1 p24 antibody [P131] (AB32352)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com