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AB308369

Anti-Nipah virus Nucleoprotein antibody [HL1436]

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(1 Publication)

Rabbit Recombinant Monoclonal Nipah virus Nucleoprotein antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Transfected cell line - Henipavirus nipahense, Transfected cell lysate - Henipavirus nipahense samples. Cited in 1 publication.
2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nipah virus Nucleoprotein antibody [HL1436] (AB308369)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nipah virus Nucleoprotein antibody [HL1436] (AB308369)

For immunofluorescence analysis, mock and transfected HEK293T cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of Nipah virus Nucleoprotein using ab308369 at 1/500 dilution (green). Blue : Fluoroshield with DAPI.

Western blot - Anti-Nipah virus Nucleoprotein antibody [HL1436] (AB308369)
  • WB

Supplier Data

Western blot - Anti-Nipah virus Nucleoprotein antibody [HL1436] (AB308369)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Nipah virus Nucleoprotein antibody [HL1436] (ab308369) diluted at 1/5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Nipah virus Nucleoprotein antibody [HL1436] (ab308369) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1436

Isotype

IgG

Carrier free

Yes

Reacts with

Henipavirus nipahense

Applications

ICC/IF, WB

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Henipavirus nipahense": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell line - Henipavirus nipahense": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Henipavirus nipahense": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NiV NP also known as Nipah nucleoprotein is one of the key structural proteins of the Nipah virus (NiV) a paramyxovirus of significant concern. The nucleoprotein has a molecular mass of approximately 55 kDa and is expressed within the host cells infected by the virus. NiV NP plays a mechanical role by encapsidating the viral RNA forming the ribonucleoprotein (RNP) complex important for the virus's replication and transcription processes. This encapsidation shields viral RNA from host cell defenses and aids in the virus's lifecycle progression.
Biological function summary

NiV NP functions in the NiV replication complex playing an essential role in the transcription and replication of the viral genome. It forms helical nucleocapsids with viral RNA working closely with other proteins such as the phosphoprotein (P) and large protein (L). NiV NP provides a scaffold for the polymerase complex and modulates its activity. It interacts with cellular mechanisms to evade the host immune response ensuring enhanced stability and survival of the virus within host cells.

Pathways

NiV NP is involved in the viral replication and transcription pathway facilitating Nipah virus's successful reproduction and spread. The nucleoprotein interacts primarily with the viral phosphoprotein (P) and plays a role in modulating the activity of the polymerase complex (Rabies P and L proteins also serve a somewhat similar function in their pathways). This involvement highlights the nucleoprotein’s critical role in maintaining the efficiency of viral genomic synthesis and transcription key steps in the viral replication cycle.

NiV NP is closely linked to the pathogenesis of Nipah virus infection which can lead to severe febrile illness and encephalitis in humans. The protein's interactions and protection of the viral genome contribute to the virus's pathogenicity. Its role in the virus's lifecycle correlates to the host's immune evasion and disease progression. NiV NP is therefore a significant target in understanding the Nipah virus's disease mechanisms and potential therapeutic interventions. Additionally its function ties with other viral proteins like the fusion protein (F) and glycoprotein (G) which together mediate cell entry and spread of the virus exacerbating the disease severity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nipah virus Nucleoprotein
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Revista do Instituto de Medicina Tropical de Sao Paulo 67:e40 PubMed40608625

2025

Nipah virus infection: preparedness for the pathological diagnosis of an emerging Paramyxoviridae disease with epidemic potential.

Applications

Unspecified application

Species

Unspecified reactive species

Cinthya Dos Santos Cirqueira Borges,Ana Maria Gonçalves,Fernanda Alencar Rodrigues,Thais de Souza Lima,Ana Catharina Seixas Nastri,Venâncio Avancini Ferreira Alves,Mirian Nacagami Sotto,Thais Mauad,Soon Hao Tan,Paulo Hilário Nascimento Saldiva,Luiz Fernando Ferraz Silva,Kum Thong Wong,Marisa Dolhnikoff,Amaro Nunes Duarte-Neto
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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