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AB245539

Anti-NIPBL antibody

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(2 Publications)

Rabbit Polyclonal NIPBL antibody. Suitable for IP and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human NIPBL aa 550 to C-terminus.

View Alternative Names

IDN3, SCC2, NIPBL, Nipped-B-like protein, Delangin, SCC2 homolog

1 Images
Immunoprecipitation - Anti-NIPBL antibody (AB245539)
  • IP

Supplier Data

Immunoprecipitation - Anti-NIPBL antibody (AB245539)

NIPBL was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (0.5 or 1 mg for IP, 20% of IP loaded) with ab245539 at 6 μg/reaction. Western blot was performed from the immunoprecipitate using a different Anti-NIPBL antibody at 1 μg/ml.

Lane 1 : ab245539 IP (Batch 1) in HeLa whole cell lysate.

Lane 2 : ab245539 IP (Batch 2) in HeLa whole cell lysate.

Lane 3 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 seconds.

Lysates prepared in NETN buffer.

All lanes:

Immunoprecipitation - Anti-NIPBL antibody (ab245539)

Predicted band size: 316 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP

applications

Immunogen

Synthetic Peptide within Human NIPBL aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6KC79

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab245539 was affinity purified using an epitope specific to NIPBL immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the loading of the cohesin complex on to DNA. Forms a heterodimeric complex (also known as cohesin loading complex) with MAU2/SCC4 which mediates the loading of the cohesin complex onto chromatin (PubMed : 22628566, PubMed : 28914604). Plays a role in cohesin loading at sites of DNA damage. Its recruitment to double-strand breaks (DSBs) sites occurs in a CBX3-, RNF8- and RNF168-dependent manner whereas its recruitment to UV irradiation-induced DNA damage sites occurs in a ATM-, ATR-, RNF8- and RNF168-dependent manner (PubMed : 28167679). Along with ZNF609, promotes cortical neuron migration during brain development by regulating the transcription of crucial genes in this process. Preferentially binds promoters containing paused RNA polymerase II. Up-regulates the expression of SEMA3A, NRP1, PLXND1 and GABBR2 genes, among others (By similarity).
See full target information NIPBL
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:206 PubMed38378967

2024

NIPBL-mediated RAD21 facilitates tumorigenicity by the PI3K pathway in non-small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoling Xu,Ding Wang,Weizhen Xu,Huihui Li,Ning Chen,Na Li,Qifeng Yao,Wei Chen,Jianxiang Zhong,Weimin Mao

NPJ precision oncology 7:28 PubMed36922568

2023

The SMARCA4 mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangwei Zeng,Bing Yao,Jianpeng Liu,Guan-Wen Gong,Ming Liu,Jiahuang Li,Hua-Feng Pan,Qixiang Li,Dongjun Yang,Peifen Lu,Dongliang Wu,Peipei Xu,Bing Chen,Panhai Chen,Ming Zhang,Ke Zen,Jian Jing,David C S Huang,Dijun Chen,Zhi-Wei Jiang,Quan Zhao
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Product promise

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