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AB254959

Anti-NIR2 antibody

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(2 Publications)

Rabbit Polyclonal NIR2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PITPNM1 aa 350-450.

View Alternative Names

DRES9, NIR2, PITPNM, PITPNM1, Membrane-associated phosphatidylinositol transfer protein 1, Drosophila retinal degeneration B homolog, Pyk2 N-terminal domain-interacting receptor 2, PITPnm 1, NIR-2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)

Immunohistochemical analysis of human cerebellum tissue labelling NIR2 in cytoplasm of Purkinje cells with ab254959 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)

Immunohistochemical analysis of human Fallopian tube tissue labelling NIR2 in cytoplasm in a subset of glandular cells with ab254959 at 1/200 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-NIR2 antibody (AB254959)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NIR2 antibody (AB254959)

PFA fixed, Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling NIR2 in cytosol and cytoplasmic bodies using ab254959 at 4μg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)

Immunohistochemical analysis of human heart muscle tissue labelling NIR2 in cytoplasm of cardiomyocytes with ab254959 at 1/200 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIR2 antibody (AB254959)

Immunohistochemical analysis of human pancreas tissue labelling NIR2 in cytoplasm in exocrine of glandular cells with ab254959 at 1/200 dilution.

Western blot - Anti-NIR2 antibody (AB254959)
  • WB

Supplier Data

Western blot - Anti-NIR2 antibody (AB254959)

All lanes:

Western blot - Anti-NIR2 antibody (ab254959) at 0.4 µg/mL

All lanes:

HDLM-2 (human Hodgkin lymphoma cell line) whole cell lysates

Predicted band size: 135 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PITPNM1 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

O00562

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the transfer of phosphatidylinositol (PI) between membranes (PubMed : 10531358, PubMed : 22822086). Binds PI, phosphatidylcholine (PC) and phosphatidic acid (PA) with the binding affinity order of PI > PA > PC (PubMed : 22822086). Regulates RHOA activity, and plays a role in cytoskeleton remodeling (PubMed : 11909959). Necessary for normal completion of cytokinesis (PubMed : 15125835). Plays a role in maintaining normal diacylglycerol levels in the Golgi apparatus (PubMed : 15723057). Necessary for maintaining the normal structure of the endoplasmic reticulum and the Golgi apparatus (PubMed : 15545272). Required for protein export from the endoplasmic reticulum and the Golgi (PubMed : 15723057). Binds calcium ions (PubMed : 10022914).
See full target information PITPNM1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 49:9424-9443 PubMed34365507

2021

SDE2 is an essential gene required for ribosome biogenesis and the regulation of alternative splicing.

Applications

Unspecified application

Species

Unspecified reactive species

Jess Floro,Anqi Dai,Abigail Metzger,Alexandra Mora-Martin,Neil J Ganem,Daniel Cifuentes,Ching-Shyi Wu,Jasbir Dalal,Shawn M Lyons,Adam Labadorf,Rachel L Flynn

Frontiers in cell and developmental biology 8:573221 PubMed33240879

2020

Inhibition of miR-490-5p Promotes Human Adipose-Derived Stem Cells Chondrogenesis and Protects Chondrocytes via the PITPNM1/PI3K/AKT Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyi Li,Xiaoyi Zhao,Xingzhao Wen,Anyu Zeng,Guping Mao,Ruifu Lin,Shu Hu,Weiming Liao,Zhiqi Zhang
View all publications
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Product promise

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