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AB273667

Anti-NIRF antibody [OTI4G10] - BSA and Azide free

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Mouse Monoclonal NIRF antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF2 aa 1-300.

View Alternative Names

NIRF, RNF107, UHRF2, E3 ubiquitin-protein ligase UHRF2, Np95/ICBP90-like RING finger protein, Nuclear protein 97, Nuclear zinc finger protein Np97, RING finger protein 107, RING-type E3 ubiquitin transferase UHRF2, Ubiquitin-like PHD and RING finger domain-containing protein 2, Ubiquitin-like-containing PHD and RING finger domains protein 2, Np95-like RING finger protein

2 Images
Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)
  • WB

Supplier Data

Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)

All lanes:

Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667) at 1/500 dilution

All lanes:

PC-3 (Human prostate adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 89 kDa

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Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)
  • WB

Supplier Data

Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)

Cells were transfected for 48 hours then lysed.

All lanes:

Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667) at 1/2000 dilution

Lane 1:

HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY NIRF cDNA at 5 µg

Predicted band size: 89 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI4G10

Isotype

IgG2a

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human UHRF2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q96PU4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from TCS.
Storage buffer
pH: 7.3 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed : 15178429, PubMed : 23404503, PubMed : 27743347, PubMed : 29506131). Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed : 24813944, PubMed : 27129234). This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity). Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed : 15178429, PubMed : 15361834, PubMed : 21952639). Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed : 12176013, PubMed : 14741369). Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation (PubMed : 29923055). Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed : 30335751). Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed : 33848395).
See full target information UHRF2
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