Mouse Monoclonal NIRF antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF2 aa 1-300.
Images
![Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667), expandable thumbnail](https://content.abcam.com/products/images/nirf-antibody-oti4g10-bsa-and-azide-free-ab273667--western-blot-img66452.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)")
![Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667), expandable thumbnail](https://content.abcam.com/products/images/nirf-antibody-oti4g10-bsa-and-azide-free-ab273667--western-blot-img54310.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (AB273667)")
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human UHRF2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PU4
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes - |
Target data
Function
E3 ubiquitin ligase that plays important roles in DNA methylation, histone modifications, cell cycle and DNA repair (PubMed:15178429, PubMed:23404503, PubMed:27743347, PubMed:29506131). Acts as a specific reader for 5-hydroxymethylcytosine (5hmC) and thereby recruits various substrates to these sites to ubiquitinate them (PubMed:24813944, PubMed:27129234). This activity also allows the maintenance of 5mC levels at specific genomic loci and regulates neuron-related gene expression (By similarity). Participates in cell cycle regulation by ubiquitinating cyclins CCND1 and CCNE1 and thereby inducing G1 arrest (PubMed:15178429, PubMed:15361834, PubMed:21952639). Ubiquitinates also PCNP leading to its degradation by the proteasome (PubMed:12176013, PubMed:14741369). Plays an active role in DNA damage repair by ubiquitinating p21/CDKN1A leading to its proteasomal degradation (PubMed:29923055). Promotes also DNA repair by acting as an interstrand cross-links (ICLs) sensor. Mechanistically, cooperates with UHRF1 to ensure recruitment of FANCD2 to ICLs, leading to FANCD2 monoubiquitination and subsequent activation (PubMed:30335751). Contributes to UV-induced DNA damage response by physically interacting with ATR in response to irradiation, thereby promoting ATR activation (PubMed:33848395).
Alternative names
NIRF, RNF107, UHRF2, E3 ubiquitin-protein ligase UHRF2, Np95/ICBP90-like RING finger protein, Nuclear protein 97, Nuclear zinc finger protein Np97, RING finger protein 107, RING-type E3 ubiquitin transferase UHRF2, Ubiquitin-like PHD and RING finger domain-containing protein 2, Ubiquitin-like-containing PHD and RING finger domains protein 2, Np95-like RING finger protein
Recommended products
Mouse Monoclonal NIRF antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human UHRF2 aa 1-300.
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.3
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human UHRF2 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q96PU4
- Carrier free
- Yes
- Clone number
- OTI4G10
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667), expandable thumbnail](https://content.abcam.com/products/images/nirf-antibody-oti4g10-bsa-and-azide-free-ab273667--western-blot-img66452.jpg "Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667)")
Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667)
NIRF Western blot staining of PC-3 (Human prostate adenocarcinoma cell line) cell extract using mouse Anti-NIRF antibody
All lanes: Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667) at 1/500 dilution
All lanes: PC-3 (Human prostate adenocarcinoma cell line) cell extract at 35 µg
Predicted band size: 89 kDa
![Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667), expandable thumbnail](https://content.abcam.com/products/images/nirf-antibody-oti4g10-bsa-and-azide-free-ab273667--western-blot-img54310.jpg "Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667)")
Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667)
NIRF Western blot staining using mouse Anti-NIRF antibody
Cells were transfected for 48 hours then lysed.
All lanes: Western blot - Anti-NIRF antibody [OTI4G10] - BSA and Azide free (ab273667) at 1/2000 dilution
Lane 1: HEK-293T cells transfected with pCMV6-ENTRY control cDNA at 5 µg
Lane 2: HEK-293T cells transfected with pCMV6-ENTRY NIRF cDNA at 5 µg
Predicted band size: 89 kDa
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