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AB180942

Anti-NIT1 antibody [EPR14577(B)]

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(1 Publication)

Rabbit Recombinant Monoclonal NIT1 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Deaminated glutathione amidase, dGSH amidase, Nitrilase homolog 1, NIT1

3 Images
Flow Cytometry (Intracellular) - Anti-NIT1 antibody [EPR14577(B)] (AB180942)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-NIT1 antibody [EPR14577(B)] (AB180942)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLacells labeling NIT1with ab180942at 1/20 dilution (red) compared to a Rabbit monoclonalIgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] (AB180942)
  • IP

Supplier Data

Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] (AB180942)

Western blot analysis of fetal liver lysate immunoprecipitated using ab180942 at 1/60 dilution (Lane 1). Lane 2 : Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG, used at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NIT1 antibody [EPR14577(B)] (ab180942)

Predicted band size: 35 kDa

false

Western blot - Anti-NIT1 antibody [EPR14577(B)] (AB180942)
  • WB

Supplier Data

Western blot - Anti-NIT1 antibody [EPR14577(B)] (AB180942)

All lanes:

Western blot - Anti-NIT1 antibody [EPR14577(B)] (ab180942) at 1/10000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 35 kDa

Observed band size: 32 kDa

false

  • Carrier free

    Anti-NIT1 antibody [EPR14577(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14577(B)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Anti-V5 tag antibody [SV5-Pk1]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/60", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/20", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the hydrolysis of the amide bond in N-(4-oxoglutarate)-L-cysteinylglycine (deaminated glutathione), a metabolite repair reaction to dispose of the harmful deaminated glutathione (PubMed : 38430071). Plays a role in cell growth and apoptosis : loss of expression promotes cell growth, resistance to DNA damage stress and increased incidence to NMBA-induced tumors. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells.
See full target information Deaminated glutathione amidase
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and translational medicine 12:e935 PubMed35834635

2022

Therapeutic induction of Bcl2-associated athanogene 3-mediated autophagy in idiopathic pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Shashipavan Chillappagari,Julian Schwarz,Vidyasagar Kesireddy,Jessica Knoell,Martina Korfei,Konrad Hoetzenecker,M Lienhard Schmitz,Christian Behl,Saverio Bellusci,Andreas Guenther,Poornima Mahavadi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com