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AB183074

Anti-NIT2 antibody

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(1 Publication)

Rabbit Polyclonal NIT2 antibody. Suitable for IHC-P, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NIT2 aa 250 to C-terminus.

View Alternative Names

CUA002, NIT2, Omega-amidase NIT2, Nitrilase homolog 2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (AB183074)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (AB183074)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling NIT2 with ab183074 at a 1/40 dilution, in the presence (right panel) or absence (left panel) of immunizing peptide. (Original magnification : ×200)

Western blot - Anti-NIT2 antibody (AB183074)
  • WB

Supplier Data

Western blot - Anti-NIT2 antibody (AB183074)

10%SDS-PAGE

All lanes:

Western blot - Anti-NIT2 antibody (ab183074) at 1/950 dilution

Lane 1:

mouse liver tissue lysate at 40 µg

Lane 2:

mouse kidney tissue lysate at 40 µg

Lane 3:

RAW264.7 cell lysate at 40 µg

Secondary

All lanes:

Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Predicted band size: 31 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human NIT2 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NQR4

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/40 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/950 - 1/5000", "WB-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Has omega-amidase activity (PubMed : 19595734, PubMed : 22674578). The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively (PubMed : 19595734).
See full target information NIT2

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 11: PubMed34573602

2021

Hypothyroidism Affects Uterine Function via the Modulation of Prostaglandin Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Kowalczyk-Zieba,Joanna Staszkiewicz-Chodor,Dorota Boruszewska,Krzysztof Lukaszuk,Joanna Jaworska,Izabela Woclawek-Potocka
View all publications

Product promise

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