AB254759

Anti-NIT2 antibody

Name: Anti-NIT2 antibody (ab254759) | Abcam
Brand: Abcam Limited
SKU: AB254759
Price: 655 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal NIT2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NIT2 aa 50-200.

View Alternative Names

CUA002, NIT2, Omega-amidase NIT2, Nitrilase homolog 2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (AB254759)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (AB254759)

Paraffin-embedded human liver tissue stained for NIT2 using ab254759 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-NIT2 antibody (AB254759)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NIT2 antibody (AB254759)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling NIT2 using ab254759 at 4µg/ml (green) in ICC/IF.

Supplier Data

Western blot - Anti-NIT2 antibody (AB254759)

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Western blot - Anti-NIT2 antibody (ab254759)

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Western blot - Anti-NIT2 antibody (ab254759) at 0.4 µg/mL

Lane 1:

MW Ladder

Lane 2:

NIH/3T3 cell lysate

Lane 3:

NBT-II cell lysate

Predicted band size: 31 kDa

false

Supplier Data

Western blot - Anti-NIT2 antibody (AB254759)

Lane 1:

Western blot - Anti-NIT2 antibody (ab254759)

Lane 2:

Western blot - Anti-NIT2 antibody (ab254759) at 0.4 µg/mL

Lane 1:

MW Ladder

Lane 2:

RT4 cell lysate

Predicted band size: 31 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NIT2 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NQR4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Has omega-amidase activity (PubMed : 19595734, PubMed : 22674578). The role of omega-amidase is to remove potentially toxic intermediates by converting 2-oxoglutaramate and 2-oxosuccinamate to biologically useful 2-oxoglutarate and oxaloacetate, respectively (PubMed : 19595734).

See full target information NIT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 21:3069-3082 PubMed39628683

2024

SLC41A1 overexpression correlates with immune cell infiltration in HCC and promotes its malignant progression.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Chen,Zhipeng Du,Caijun Rao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NIT2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NIT2 antibody (AB254759)

Immunocytochemistry/ Immunofluorescence - Anti-NIT2 antibody (AB254759)

Western blot - Anti-NIT2 antibody (AB254759)

Western blot - Anti-NIT2 antibody (AB254759)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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