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AB53232

Anti-Nitrotyrosine antibody [11C2]

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(10 Publications)

Mouse Monoclonal Nitrotyrosine antibody. Suitable for ICC, ELISA, WB and reacts with Chemical samples. Cited in 10 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Nitrotyrosine.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

11C2

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Applications

WB, ELISA, ICC

applications

Specificity

This antibody reacts with free and bound Nitrotyrosine.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "1/100 - 1/500", "ICC-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/100 - 1/1000", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.02% Sodium azide Constituents: Tissue culture supernatant
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.
Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nitrotyrosine
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Biochemical pharmacology 220:115978 PubMed38081369

2023

Unlocking the protective potential of the angiotensin type 2 receptor (ATR) in acute lung injury and age-related pulmonary dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Peter Abadir,Caglar Cosarderelioglu,Mahendra Damarla,Alla Malinina,Dustin Dikeman,Ruth Marx,Monica M Nader,Michael Abadir,Jeremy Walston,Enid Neptune

Frontiers in cell and developmental biology 10:836035 PubMed35356291

2022

Isolevuglandins Scavenger Ameliorates Myocardial Ischemic Injury by Suppressing Oxidative Stress, Apoptosis, and Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Guo,Fengqiang Xu,Hongwei Ji,Yajun Jing,Li Shen,Xinyu Weng,Longgang Hu

Frontiers in pharmacology 13:824138 PubMed35350766

2022

Cajaninstilbene Acid Ameliorates Acetaminophen-Induced Liver Injury Through Enhancing Sestrin2/AMPK-Mediated Mitochondrial Quality Control.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Yan,Suwei Jin,Yongguang Liu,Lisha Wang,Zhi Wang,Tianji Xia,Qi Chang

Journal of cellular physiology 237:897-910 PubMed34459512

2021

Trimethylamine N-oxide exacerbates acetaminophen-induced liver injury by interfering with macrophage-mediated liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Yan,Chong Zhao,Shangyun Lu,Jinling Cui,Zhenou Sun,Xiaoyi Liu,Shuo Liu,Yazhen Huo,Shutao Yin,Hongbo Hu

Cell death & disease 10:3 PubMed30584234

2018

Lack of the brain-specific isoform of apoptosis-inducing factor aggravates cerebral damage in a model of neonatal hypoxia-ischemia.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Rodriguez,Yaodong Zhang,Tao Li,Cuicui Xie,Yanyan Sun,Yiran Xu,Kai Zhou,Kaiming Huo,Yafeng Wang,Xiaoyang Wang,Daniel Andersson,Anders Ståhlberg,Qinghe Xing,Carina Mallard,Henrik Hagberg,Nazanine Modjtahedi,Guido Kroemer,Klas Blomgren,Changlian Zhu

British journal of pharmacology 175:3747-3757 PubMed30024038

2018

Glycycoumarin protects mice against acetaminophen-induced liver injury predominantly via activating sustained autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Yan,Linhu Ye,Shutao Yin,Xiaotong Lu,Xiaoyi Liu,Shangyun Lu,Jinling Cui,Lihong Fan,Neil Kaplowitz,Hongbo Hu

EMBO molecular medicine 4:660-73 PubMed22488900

2012

Modification of γ-secretase by nitrosative stress links neuronal ageing to sporadic Alzheimer's disease.

Applications

WB

Species

Unspecified reactive species

Francesc X Guix,Tina Wahle,Kristel Vennekens,An Snellinx,Lucía Chávez-Gutiérrez,Gerard Ill-Raga,Eva Ramos-Fernandez,Cristina Guardia-Laguarta,Alberto Lleó,Muriel Arimon,Oksana Berezovska,Francisco J Muñoz,Carlos G Dotti,Bart De Strooper

The American journal of pathology 175:84-96 PubMed19541933

2009

Complex integration of matrix, oxidative stress, and apoptosis in genetic emphysema.

Applications

IHC-P

Species

Unspecified reactive species

Megan Podowski,Carla L Calvi,Christopher Cheadle,Rubin M Tuder,Shyam Biswals,Enid R Neptune

American journal of physiology. Lung cellular and molecular physiology 296:L565-73 PubMed19151108

2009

Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia.

Applications

IHC-P

Species

Unspecified reactive species

Sharon McGrath-Morrow,Thomas Lauer,Min Yee,Enid Neptune,Megan Podowski,Rajesh K Thimmulappa,Michael O'Reilly,Shyam Biswal

The journal of sexual medicine 4:908-16 PubMed17627738

2007

FK506 and sildenafil promote erectile function recovery after cavernous nerve injury through antioxidative mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Gwen Lagoda,Liming Jin,Todd J Lehrfeld,Tongyun Liu,Arthur L Burnett
View all publications
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