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AB125106

Anti-Nitrotyrosine antibody [EM-30]

5

(3 Reviews)

|

(11 Publications)

Mouse Monoclonal Nitrotyrosine antibody. Suitable for IHC-Fr, IHC-P, WB and reacts with Chemical samples. Cited in 11 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Nitrotyrosine.
1 Images
Immunohistochemistry (Frozen sections) - Anti-Nitrotyrosine antibody [EM-30] (AB125106)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Nitrotyrosine antibody [EM-30] (AB125106)

Immunohistochemical analysis of Human tumoral colon epithelium (frozen sections) labelling Nitrotyrosine with ab125106 at 10 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

EM-30

Isotype

IgG1

Carrier free

No

Applications

IHC-Fr, WB, IHC-P

applications

Specificity

Detects nitrotyrosine group in biological material. It can beused as a marker for peroxynitrite formation in particular tissues.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "10 µg/mL", "IHCFr-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Purified from cell culture supernatant by affinity chromatography.Purity is > 95% (by SDS-PAGE).
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nitrotyrosine also known as 3-nitrotyrosine is a marker for nitrosative stress and oxidative damage. It comes from the nitration of tyrosine an amino acid primarily through the action of reactive nitrogen species. Nitrotyrosine can be found in proteins and its molecular mass varies depending on the protein context. It is present across various tissues and fluid samples within the body particularly where high oxidative stress occurs.
Biological function summary

Nitrotyrosine acts as an indicator of cellular damage and protein modification due to stress conditions. When proteins have nitrotyrosine residues their function might change which can lead to cellular signaling disruption. Nitrotyrosine is not usually part of a specific complex but it affects proteins and enzymes by altering their activity and stability contributing to larger stress response mechanisms.

Pathways

Proteins modified by nitrotyrosine are involved in cellular pathways related to oxidative stress and inflammation. Particularly pathways like the nitric oxide synthase pathway and the inflammatory response pathway include nitrotyrosine-modified proteins. It is related to proteins such as nitric oxide synthase (NOS) and superoxide dismutase (SOD) important in managing oxidative balance and stress responses in cells.

Nitrotyrosine is significantly linked to diseases related to oxidative stress such as cardiovascular disease and neurodegenerative disorders. These conditions often have increased levels of nitrotyrosine indicating heightened oxidative damage. Through these diseases nitrotyrosine connects with proteins like amyloid-beta in neurodegenerative disorders and endothelial nitric oxide synthase (eNOS) in cardiovascular diseases reflecting the impact of oxidative stress on these proteins.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Nitrotyrosine
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular biosciences 12:1637230 PubMed40746423

2025

Development of a human colorectal carcinoma cell-based platform for studying inducible nitric oxide synthase expression and nitric oxide signaling dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Elizabeth A Grimm,Yong Qin

PloS one 19:e0310394 PubMed39570915

2024

Cardiac effects of OPA1 protein promotion in a transgenic animal model.

Applications

Unspecified application

Species

Unspecified reactive species

Kitti Bruszt,Orsolya Horvath,Katalin Ordog,Szilard Toth,Kata Juhasz,Eszter Vamos,Katalin Fekete,Ferenc Gallyas,Kalman Toth,Robert Halmosi,Laszlo Deres

International journal of molecular sciences 25: PubMed39062895

2024

Time-Restricted Feeding Protects against Renal Ischemia-Reperfusion Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Do Kyun Kim,Young Suk Kim,Min Jeong Kim,Seo Rin Kim,Dong Won Lee,Soo Bong Lee,Il Young Kim

International journal of molecular sciences 25: PubMed39063073

2024

Vascular Endothelial Growth Factor C (VEGF-C) Sensitizes Lymphatic Endothelial Cells to Oxidative-Stress-Induced Apoptosis through DNA Damage and Mitochondrial Dysfunction: Implications for Lymphedema.

Applications

Unspecified application

Species

Unspecified reactive species

Lazina Hossain,Karina Pereira Gomes,Xiaoyan Yang,Emily Liu,Jacques Du Toit,Pierre-Yves von der Weid,Spencer Bruce Gibson

Kidney research and clinical practice 43:444-456 PubMed38934035

2024

Time-restricted feeding protects against cisplatin-induced acute kidney injury in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kyu Won Jang,Young Suk Kim,Min Jeong Kim,Seo Rin Kim,Dong Won Lee,Soo Bong Lee,Il Young Kim

eNeuro 10: PubMed36635251

2023

The Neurotoxin DSP-4 Dysregulates the Locus Coeruleus-Norepinephrine System and Recapitulates Molecular and Behavioral Aspects of Prodromal Neurodegenerative Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Alexa F Iannitelli,Michael A Kelberman,Daniel J Lustberg,Anu Korukonda,Katharine E McCann,Bernard Mulvey,Arielle Segal,L Cameron Liles,Steven A Sloan,Joseph D Dougherty,David Weinshenker

Scientific reports 10:12368 PubMed32704023

2020

Soluble Klotho, a biomarker and therapeutic strategy to reduce bronchopulmonary dysplasia and pulmonary hypertension in preterm infants.

Applications

Unspecified application

Species

Unspecified reactive species

Sunil Batlahally,Andrew Franklin,Andreas Damianos,Jian Huang,Pingping Chen,Mayank Sharma,Joanne Duara,Divya Keerthy,Ronald Zambrano,Lina A Shehadeh,Eliana C Martinez,Marissa J DeFreitas,Shathiyah Kulandavelu,Carolyn L Abitbol,Michael Freundlich,Rosemeire M Kanashiro-Takeuchi,Augusto Schmidt,Merline Benny,Shu Wu,Karen K Mestan,Karen C Young

Environmental science and pollution research inter 26:21556-21564 PubMed31127514

2019

Could curcumin ameliorate titanium dioxide nanoparticles effect on the heart? A histopathological, immunohistochemical, and genotoxic study.

Applications

Unspecified application

Species

Unspecified reactive species

Eman Ahmed Alaa El-Din,Heba El-Sayed Mostafa,Mai A Samak,Eman M Mohamed,Dalia Abdallah El-Shafei

BMC nephrology 19:248 PubMed30253743

2018

The two isoforms of matrix metalloproteinase- 2 have distinct renal spatial and temporal distributions in murine models of types 1 and 2 diabetes mellitus.

Applications

Unspecified application

Species

Unspecified reactive species

Il Young Kim,Sang Soo Kim,Hye Won Lee,Sun Sik Bae,Hong Koo Ha,Eun Soon Jung,Min Young Lee,Miyeun Han,Harin Rhee,Eun Young Seong,Dong Won Lee,Soo Bong Lee,David H Lovett,Sang Heon Song

IUBMB life 69:188-201 PubMed28164440

2017

Effect of human umbilical cord blood mesenchymal stem cells administered by intravenous or intravitreal routes on cryo-induced retinal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Eman M Mohamed,Shaimaa A Abdelrahman,Samia Hussein,Sally M Shalaby,Hala Mosaad,Ahmed M B Awad
View all publications
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