AB61705

Anti-NK-1R antibody

Name: Anti-NK-1R antibody (ab61705) | Abcam
Brand: Abcam Limited
SKU: AB61705
Price: 535 USD
Availability: InStock
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Goat Polyclonal NK-1R antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TACR1 aa 200-250.

View Alternative Names

NK1R, TAC1R, TACR1, Substance-P receptor, SPR, NK-1 receptor, Tachykinin receptor 1, NK-1R

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (AB61705)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (AB61705)

Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized Neuro2a (Mouse neuroblastoma cell line) cells labelling NK-1R with primary Anti-NK-1R antibody (ab61705) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml.

Supplier Data

Western blot - Anti-NK-1R antibody (AB61705)

Lane 1-2 : ab61705 (1µg/ml) staining of HeLa (A) and (0.5ug/ml) Neuro2a (B) cell lysate. Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.

All lanes:

Western blot - Anti-NK-1R antibody (ab61705)

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 35 µg

Lane 2:

Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 35 µg

Predicted band size: 46 kDa

Observed band size: 48 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human TACR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

P25103

Specificity

This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "1 hour primary incubation is recommended for this product.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the tachykinin substance P, also able to bind and respond to tachynins neurokinin A/substance K and neurokinin B/neuromedin-K (PubMed : 1718267, PubMed : 15452552). The rank order of affinity of this receptor to tachykinins is : substance P > neurokinin A/substance K > neurokinin B/neuromedin-K (PubMed : 1718267). Substance P binding to its receptor triggers G protein-coupled receptor signaling via activation of phosphatidylinositol hydrolysis by phospholipase C. Substance P binding also triggers signaling via activation of adenylate cyclase activity which results in increased intracellular levels of cyclic AMP (cAMP) (By similarity).

See full target information TACR1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Stem cell reports 17:849-863 PubMed35334220

2022

Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Lasagni Vitar,Francesca Triani,Marco Barbariga,Philippe Fonteyne,Paolo Rama,Giulio Ferrari

Investigative ophthalmology & visual science 62:21 PubMed34698773

2021

A Hypothalamic-Controlled Neural Reflex Promotes Corneal Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Mayra Lasagni Vitar,Philippe Fonteyne,Linda Chaabane,Paolo Rama,Giulio Ferrari

Investigative ophthalmology & visual science 62:26 PubMed33729475

2021

Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade.

Applications

Unspecified application

Species

Unspecified reactive species

Romina Mayra Lasagni Vitar,Marco Barbariga,Philippe Fonteyne,Fabio Bignami,Paolo Rama,Giulio Ferrari

Artificial cells, nanomedicine, and biotechnology 47:1628-1634 PubMed31010320

2019

Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Liu,Yuelan Zhu,Wei Zheng,Tangliang Qian,Haiyu Wang,Xiujuan Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NK-1R antibody

Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (AB61705)

Western blot - Anti-NK-1R antibody (AB61705)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise