Goat Polyclonal NK-1R antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TACR1 aa 200-250.
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Publications
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- Stem cell reports 17:849-8632022Substance P/neurokinin-1 receptor pathway blockade ameliorates limbal stem cell deficiency by modulating mTOR pathway and preventing cell senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomina Lasagni Vitar,Francesca Triani,Marco Barbariga,Philippe Fonteyne,Paolo Rama,Giulio FerrariPubMed 35334220
- Investigative ophthalmology & visual science 62:212021A Hypothalamic-Controlled Neural Reflex Promotes Corneal Inflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomina Mayra Lasagni Vitar,Philippe Fonteyne,Linda Chaabane,Paolo Rama,Giulio FerrariPubMed 34698773
- Investigative ophthalmology & visual science 62:262021Modulating Ocular Surface Pain Through Neurokinin-1 Receptor Blockade.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRomina Mayra Lasagni Vitar,Marco Barbariga,Philippe Fonteyne,Fabio Bignami,Paolo Rama,Giulio FerrariPubMed 33729475
- Artificial cells, nanomedicine, and biotechnology 47:1628-16342019Antagonism of NK-1R using aprepitant suppresses inflammatory response in rheumatoid arthritis fibroblast-like synoviocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoping Liu,Yuelan Zhu,Wei Zheng,Tangliang Qian,Haiyu Wang,Xiujuan HouPubMed 31010320
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TACR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P25103
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Tested |
| Dog | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
Species Mouse | Dilution info 0.30000-1.00000 µg/mL | Notes 1 hour primary incubation is recommended for this product. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
This is a receptor for the tachykinin neuropeptide substance P. It is probably associated with G proteins that activate a phosphatidylinositol-calcium second messenger system. The rank order of affinity of this receptor to tachykinins is: substance P > substance K > neuromedin-K.
Alternative names
NK1R, TAC1R, TACR1, Substance-P receptor, SPR, NK-1 receptor, Tachykinin receptor 1, NK-1R
Recommended products
Goat Polyclonal NK-1R antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TACR1 aa 200-250.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TACR1 aa 200-250. The exact immunogen used to generate this antibody is proprietary information. Database link P25103
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody is expected to recognise both reported isoforms (NP_001049.1 and NP_056542.1).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-NK-1R antibody (ab61705)
Immunofluorescence analysis of paraformaldehyde fixed, 0.15% Triton permeabilized Neuro2a (Mouse neuroblastoma cell line) cells labelling NK-1R with primary Anti-NK-1R antibody (ab61705) at 10ug/ml for 1 hour, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. Image showing nuclear and plasma membrane staining. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG at 10ug/ml followed by Alexa Fluor 488 secondary antibody at 2ug/ml.
Western blot - Anti-NK-1R antibody (ab61705)
Lane 1-2: ab61705 (1µg/ml) staining of HeLa (A) and (0.5ug/ml) Neuro2a (B) cell lysate.
Primary incubation 1 hour at room temperature. Detected by chemiluminescence. RIPA buffer used.All lanes: Western blot - Anti-NK-1R antibody (ab61705)
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 35 µg
Lane 2: Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 35 µg
Predicted band size: 46 kDa
Observed band size: 48 kDa
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