AB133668

Anti-NK-p44 antibody [EPR5060(2)]

RabMAb
Recombinant
What is this?

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(3 Publications)

Rabbit Recombinant Monoclonal NK-p44 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.

View Alternative Names

CD336, LY95, NCR2, Natural cytotoxicity triggering receptor 2, Lymphocyte antigen 95 homolog, NK cell-activating receptor, Natural killer cell p44-related protein, NK-p44, NKp44

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

Immunofluorescent analysis of K562 cells labelling NK-p44 with ab133668 at 1/100 dilution.

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Western blot - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

All lanes:

Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668) at 1/10000 dilution

Lane 1:

Human lymph node lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HuT-78 cell lysate at 10 µg

Lane 5:

Molt-4 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugate at 1/2000 dilution

Predicted band size: 31 kDa

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OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

OI-RD Scanning

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OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-NK-p44 antibody [EPR5060(2)] - BSA and Azide free
Carrier free

Anti-NK-p44 antibody [EPR5060(2)] - Low endotoxin, Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5060(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.

See full target information NCR2

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular biochemistry 458:159-169 PubMed31020492

2019

Biological effects of cyclosporin A on CD3CD161 and CD3CD161 lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Aravinthan Adithan,Judith Sharmila John Peter,Mohammad Amjad Hossain,Chang-Won Kang,Bumseok Kim,Nam Soo Kim,Ki-Chul Hwang,Jong-Hoon Kim

International journal of molecular sciences 18: PubMed29137183

2017

Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqin Qiu,Zongyong Jiang,Shenglan Hu,Li Wang,Xianyong Ma,Xuefen Yang

Frontiers in immunology 8:1283 PubMed29067027

2017

Exoenzyme T Plays a Pivotal Role in the IFN-γ Production after Challenge in IL-12 Primed Natural Killer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Mickael Vourc'h,Antoine Roquilly,Alexis Broquet,Gaelle David,Philippe Hulin,Cedric Jacqueline,Jocelyne Caillon,Christelle Retiere,Karim Asehnoune

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NK-p44 antibody [EPR5060(2)]

Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

Western blot - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (3)

Product promise