Rabbit Recombinant Monoclonal NK-p44 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
Images
![Western blot - Anti-NK-p44 antibody [EPR5060(2)] (AB133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--western-blot-img111951.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)")
![Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (AB133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--immunocytochemistry-immunofluorescence-img120962.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)")
![OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (AB133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--oi-rd-scanning-img139128.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (AB133668)")
Publications
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- Molecular and cellular biochemistry 458:159-1692019Biological effects of cyclosporin A on CD3CD161 and CD3CD161 lymphocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAravinthan Adithan et. al.PubMed 31020492
- International journal of molecular sciences 18:2017Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYueqin Qiu et. al.PubMed 29137183
- Frontiers in immunology 8:12832017Exoenzyme T Plays a Pivotal Role in the IFN-γ Production after Challenge in IL-12 Primed Natural Killer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMickael Vourc'h et. al.PubMed 29067027
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Predicted | Not recommended | Predicted |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 - 1/50000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis.
Alternative names
CD336, LY95, NCR2, Natural cytotoxicity triggering receptor 2, Lymphocyte antigen 95 homolog, NK cell-activating receptor, Natural killer cell p44-related protein, NK-p44, NKp44
Recommended products
Rabbit Recombinant Monoclonal NK-p44 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR5060(2)
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 1 x 10-10 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--western-blot-img111951.jpg "Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)")
Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
All lanes: Western blot - Anti-NK-p44 antibody [EPR5060(2)] (ab133668) at 1/10000 dilution
Lane 1: Human lymph node lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: HuT-78 cell lysate at 10 µg
Lane 5: Molt-4 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugate at 1/2000 dilution
Predicted band size: 31 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--immunocytochemistry-immunofluorescence-img120962.jpg "Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)")
Immunocytochemistry/ Immunofluorescence - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
Immunofluorescent analysis of K562 cells labelling NK-p44 with ab133668 at 1/100 dilution.
![OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668), expandable thumbnail](https://content.abcam.com/products/images/nk-p44-antibody-epr50602-ab133668--oi-rd-scanning-img139128.jpg "OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)")
OI-RD Scanning - Anti-NK-p44 antibody [EPR5060(2)] (ab133668)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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