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Rabbit Polyclonal NKB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TAC3 aa 1-100.

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Images

Western blot - Anti-NKB antibody (AB244479), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKB antibody (AB244479), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-NKB antibody (AB244479), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human TAC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHF0

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBICC/IF
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/500.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
0.04000-0.40000 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
0.25000-2.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Tachykinins are active peptides which excite neurons, evoke behavioral responses, are potent vasodilators and secretagogues, and contract (directly or indirectly) many smooth muscles (By similarity). Is a critical central regulator of gonadal function.

Alternative names

Recommended products

Rabbit Polyclonal NKB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human TAC3 aa 1-100.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human TAC3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UHF0
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-NKB antibody (ab244479), expandable thumbnail

    Western blot - Anti-NKB antibody (ab244479)

    All lanes: Western blot - Anti-NKB antibody (ab244479) at 0.4 µg/mL

    Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lane 2: NKB (with C-terminal myc-DDK tag) everexpressed in HEK-293T, cell lysate

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKB antibody (ab244479), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKB antibody (ab244479)

    Paraffin-embedded human cerebellum tissue stained for NKB using ab244479 at 1/200 dilution in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-NKB antibody (ab244479), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-NKB antibody (ab244479)

    PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for NKB (green) using ab244479 at 4 μg/ml in ICC/IF.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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