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AB99558

Anti-NKCC1 antibody

3

(1 Review)

|

(4 Publications)

Goat Polyclonal NKCC1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human SLC12A2 aa 750-800.

View Alternative Names

NKCC1, SLC12A2, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Na-K-2Cl cotransporter 1, BSC2, hNKCC1

1 Images
Flow Cytometry - Anti-NKCC1 antibody (AB99558)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKCC1 antibody (AB99558)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/ml) followed by Alexa Fluor 488 secondary antibody (1µg/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Human SLC12A2 aa 750-800. The exact immunogen used to generate this antibody is proprietary information.

P55011

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cynomolgus monkey": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Ammonium sulphate precipitation followed by antigen affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane (PubMed : 16669787, PubMed : 32081947, PubMed : 32294086, PubMed : 33597714, PubMed : 35585053, PubMed : 36239040, PubMed : 36306358, PubMed : 7629105). Plays a vital role in the regulation of ionic balance and cell volume (PubMed : 16669787, PubMed : 32081947, PubMed : 32294086, PubMed : 7629105).
See full target information SLC12A2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of biological rhythms 35:275-286 PubMed32406304

2020

The Excitatory Effects of GABA within the Suprachiasmatic Nucleus: Regulation of Na-K-2Cl Cotransporters (NKCCs) by Environmental Lighting Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

John K McNeill,James C Walton,Vitaly Ryu,H Elliott Albers

Frontiers in neuroscience 14:31 PubMed32082109

2020

Asthmatic Airway Vagal Hypertonia Involves Chloride Dyshomeostasis of Preganglionic Neurons in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ding He,Hong Chen,Ming Zeng,Chunmei Xia,Jin Wang,Linlin Shen,Danian Zhu,Yonghua Chen,Jijiang Wang

eLife 8: PubMed31746738

2019

Seasonal plasticity in GABA signaling is necessary for restoring phase synchrony in the master circadian clock network.

Applications

Unspecified application

Species

Unspecified reactive species

Kayla E Rohr,Harshida Pancholi,Shabi Haider,Christopher Karow,David Modert,Nicholas J Raddatz,Jennifer Evans

Journal of cellular physiology 234:1630-1642 PubMed30159893

2018

NKCC1 promotes EMT-like process in GBM via RhoA and Rac1 signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Haiwen Ma,Tao Li,Zhennan Tao,Long Hai,Luqing Tong,Li Yi,Iruni R Abeysekera,Peidong Liu,Yang Xie,Jiabo Li,Feng Yuan,Chen Zhang,Yihan Yang,Haolang Ming,Shengping Yu,Xuejun Yang
View all publications
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Product promise

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