Anti-NKCC1 antibody [EPR26256-60]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Recombinant
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(4 Publications)
Anti-NKCC1 antibody [EPR26256-60] (ab303518) is a rabbit monoclonal antibody detecting NKCC1 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
View Alternative Names
NKCC1, SLC12A2, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, Na-K-2Cl cotransporter 1, BSC2, hNKCC1
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NKCC1 with ab303518 at 1/100 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on human colon(PMID : 17478539).The section was incubated with ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T84 (human colon epithelial cell) cells labelling NKCC1 with ab303518 at 1/100 (4.89 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly membranous staining in T84 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T84 (human colon epithelial cell) cells labelling NKCC1 with ab303518 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NKCC1 with ab303518 at 1/100 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Negative control : No staining on human liver.The section was incubated with ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IP
Supplier Data
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
NKCC1 was immunoprecipitated from 0.35 mg human cerebellum tissue lysate 10 ug with ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303518. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : human cerebellum tissue lysate 10 µg (Inset) Lane 2 : ab303518 IP in human cerebellum tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303518 in human cerebellum tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 minutes
All lanes:
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/30 dilution
Lane 1:
human cerebellum tissue lysate at 10 µg
Lane 2:
ab303518 IP in human cerebellum tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Predicted band size: 131 kDa
Observed band size: 150 kDa
false
Exposure time: 3min
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NKCC1 with ab303518 at 1/2000 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on rat colon.The section was incubated with ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- IHC-P
Supplier Data
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling NKCC1 with ab303518 at 1/2000 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond Polymer Refine Detection) was used. Positive staining on mouse colon.The section was incubated with ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NKCC1 with ab303518 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NKCC1 with ab303518 at 1/100 (4.89 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in NIH/3T3 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
- IP
Supplier Data
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
NKCC1 was immunoprecipitated from 0.35 mg rat brain tissue lysate 10 ug with ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303518. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution. Lane 1 : rat brain tissue lysate 10 µg (Inset) Lane 2 : ab303518 IP in rat brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303518 in rat brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 23 seconds.
All lanes:
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/30 dilution
Lane 1:
Rat brain tissue lysate at 10 µg
Lane 2:
ab303518 IP in rat brain tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Predicted band size: 131 kDa
Observed band size: 150 kDa
false
Exposure time: 23s
- IP
Supplier Data
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
NKCC1 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 ug with ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab303518 at dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : mouse brain tissue lysate 10 µg (Inset) Lane 2 : ab303518 IP in mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab303518 in mouse brain tissue lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 15 seconds.
All lanes:
Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/30 dilution
Lane 1:
Mouse brain tissue lysate at 10 µg
Lane 2:
ab303518 IP in mouse brain tissue lysate
Secondary
All lanes:
Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution
Predicted band size: 131 kDa
Observed band size: 150 kDa
false
Exposure time: 15s
- WB
Supplier Data
Western blot - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Blocking / diluting buffer and concentration : 5% NFDM/TBST Low expression : liver (human protein atlas database)
All lanes:
Western blot - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/1000 dilution
Lane 1:
Human cerebellum tissue lysate at 20 µg
Lane 2:
Human liver tissue lysate at 20 µg
Lane 3:
Mouse brain tissue lysate at 20 µg
Lane 4:
Mouse colon cancer tissue lysate at 20 µg
Lane 5:
Mouse liver tissue lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human Ig at 1/2000 dilution
Predicted band size: 131 kDa
Observed band size: 150 kDa
false
Exposure time: 3s
- WB
Supplier Data
Western blot - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Blocking / diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/1000 dilution
All lanes:
Rat brain tissue lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 131 kDa
Observed band size: 150 kDa
false
Exposure time: 8s
- WB
Supplier Data
Western blot - Anti-NKCC1 antibody [EPR26256-60] (AB303518)
Blocking / diluting buffer and concentration : 5% NFDM/TBST This antibody does not cross-react with mouse NKCC2.
All lanes:
Western blot - Anti-NKCC1 antibody [EPR26256-60] (ab303518) at 1/1000 dilution
Lane 1:
His-tagged mouse NKCC2 recombinant protein at 10 ng
Lane 2:
His-tagged mouse NKCC1 recombinant protein at 10 ng
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution
Predicted band size: 131 kDa
Observed band size: 30 kDa
false
Exposure time: 26s
Related conjugates and formulations (1)
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Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free
Reactivity data
Product details
What is this antibody validated in?
Anti-NKCC1 antibody [EPR26256-60] (ab303518) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of NKCC1?
Anti-NKCC1 [EPR26256-60] (ab303518) specifically detects a band for NKCC1 (UniProt: P55012) at a molecular weight of 131kDa.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR26256-60] also available for your convenience: ab303518, Carrier free - ab303519
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
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Target data
Publications (4)
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Frontiers in pharmacology 16:1589143 PubMed40880648
2025
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Acta pharmaceutica Sinica. B 14:4544-4559 PubMed39525574
2024
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Unspecified reactive species
Brain, behavior, & immunity - health 42:100890 PubMed39507306
2024
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Unspecified application
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Unspecified reactive species
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2306622 PubMed38353402
2024
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Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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