Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free

14 product images

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  Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  NKCC1 was immunoprecipitated from 0.35 mg human cerebellum tissue lysate 10 ug with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-NKCC1 antibody [EPR26256-60] ab303518. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: human cerebellum tissue lysate 10 µg (Inset)
  
  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in human cerebellum tissue lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NKCC1 antibody [EPR26256-60] ab303518 in human cerebellum tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 3 minutes

  All lanes: Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/30 dilution

  Lane 1: human cerebellum tissue lysate at 10 µg

  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in human cerebellum tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Predicted band size: 131 kDa

  Observed band size: 150 kDa

  Exposure time: 3min

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  Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  NKCC1 was immunoprecipitated from 0.35 mg rat brain tissue lysate 10 ug with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-NKCC1 antibody [EPR26256-60] ab303518. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: rat brain tissue lysate 10 µg (Inset)
  
  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in rat brain tissue lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NKCC1 antibody [EPR26256-60] ab303518 in rat brain tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 23 seconds.

  All lanes: Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/30 dilution

  Lane 1: Rat brain tissue lysate at 10 µg

  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in rat brain tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Predicted band size: 131 kDa

  Observed band size: 150 kDa

  Exposure time: 23s

• 

  Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  NKCC1 was immunoprecipitated from 0.35 mg mouse brain tissue lysate 10 ug with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using Anti-NKCC1 antibody [EPR26256-60] ab303518 at dilution. VeriBlot for IP secondary antibody (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
  
  Lane 1: mouse brain tissue lysate 10 µg (Inset)
  
  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in mouse brain tissue lysate
  
  Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-NKCC1 antibody [EPR26256-60] ab303518 in mouse brain tissue lysate
  
  Blocking and dilution buffer and concentration: 5% NFDM/TBST.
  
  Exposure time: 15 seconds.

  All lanes: Immunoprecipitation - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/30 dilution

  Lane 1: Mouse brain tissue lysate at 10 µg

  Lane 2: Anti-NKCC1 antibody [EPR26256-60] ab303518 IP in mouse brain tissue lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Predicted band size: 131 kDa

  Observed band size: 150 kDa

  Exposure time: 15s

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  Western blot - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  Blocking / diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Western blot - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/1000 dilution

  All lanes: Rat brain tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 131 kDa

  Observed band size: 150 kDa

  Exposure time: 8s

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  Western blot - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  Blocking / diluting buffer and concentration: 5% NFDM/TBST
  
  Low expression: liver (human protein atlas database)

  All lanes: Western blot - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/1000 dilution

  Lane 1: Human cerebellum tissue lysate at 20 µg

  Lane 2: Human liver tissue lysate at 20 µg

  Lane 3: Mouse brain tissue lysate at 20 µg

  Lane 4: Mouse colon cancer tissue lysate at 20 µg

  Lane 5: Mouse liver tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human Ig at 1/2000 dilution

  Predicted band size: 131 kDa

  Observed band size: 150 kDa

  Exposure time: 3s

• 

  Western blot - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  Blocking / diluting buffer and concentration: 5% NFDM/TBST
  
  This antibody does not cross-react with mouse NKCC2.

  All lanes: Western blot - Anti-NKCC1 antibody [EPR26256-60] (Anti-NKCC1 antibody [EPR26256-60] ab303518) at 1/1000 dilution

  Lane 1: His-tagged mouse NKCC2 recombinant protein at 10 ng

  Lane 2: His-tagged mouse NKCC1 recombinant protein at 10 ng

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

  Predicted band size: 131 kDa

  Observed band size: 30 kDa

  Exposure time: 26s

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  Flow Cytometry - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Flow Cytometry - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.
  
  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized T84 (human colon epithelial cell) cells labelling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody.

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  Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized NIH/3T3 (mouse embryonic fibroblast) cells labelling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/100 (4.89 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membranous and cytoplasmic staining in NIH/3T3 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized T84 (human colon epithelial cell) cells labelling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/100 (4.89 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing mainly membranous staining in T84 cell line. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/100 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control: No staining on human liver.The section was incubated with Anti-NKCC1 antibody [EPR26256-60] ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/2000 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat colon.The section was incubated with Anti-NKCC1 antibody [EPR26256-60] ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/2000 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse colon.The section was incubated with Anti-NKCC1 antibody [EPR26256-60] ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKCC1 antibody [EPR26256-60] - BSA and Azide free (ab303519)

  This data was developed using Anti-NKCC1 antibody [EPR26256-60] ab303518, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling NKCC1 with Anti-NKCC1 antibody [EPR26256-60] ab303518 at 1/100 (4.89 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human colon(PMID: 17478539).The section was incubated with Anti-NKCC1 antibody [EPR26256-60] ab303518 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins