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AB171243

Anti-NKG2D antibody [A10] - Low endotoxin, Azide free

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(1 Publication)

Armenian hamster Monoclonal NKG2D antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD314, D12S2489E, NKG2D, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor

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Flow Cytometry - Anti-NKG2D antibody [A10] - Low endotoxin, Azide free (AB171243)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2D antibody [A10] - Low endotoxin, Azide free (AB171243)

Flow Cytometric analysis staining Mouse splenocytes with an anti-Mouse CD49b (Integrin alpha 2) APC antibody and the PE conjugated version of ab171243. Total viable cells were used for analysis.

Key facts

Host species

Armenian hamster

Clonality

Monoclonal

Clone number

A10

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

Flow Cyt

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p>ab171243 can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10e5 to 10e8 cells/test.</p><p><a href='/en-us/products/primary-antibodies/armenian-hamster-igg-monoclonal-htk888-isotype-control-ab18479'>ab18479</a> - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Endotoxin Level: Less than 0.001 ng/ug antibody, as determined by the LAL assay.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7.2
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKG2D also known as natural killer group 2 member D is a type-2 transmembrane receptor with a mass of approximately 42 kDa. Expressed primarily on natural killer (NK) cells CD8+ T cells subsets of CD4+ T cells and some macrophages it recognizes stress-induced ligands presented on target cells. This protein plays an important role in the immune system's recognition and elimination of infected or transformed cells by binding to its ligands which include MHC class I chain-related proteins MICA/B and UL16-binding proteins.
Biological function summary

NKG2D acts as an activating receptor on the cell surface that initiates immune responses leading to cytotoxicity against target cells. It functions as part of a complex involving the adaptor protein DAP10 which transduces activating signals to promote cellular immune responses. This interaction amplifies the activation of immune cells and contributes to the host's defense against tumors and virally infected cells.

Pathways

NKG2D plays a significant role in immune surveillance and signaling pathways. It actively involves in the natural killer (NK) cell-mediated cytotoxicity pathway and the adaptive immune response pathway. The interaction with proteins such as DAP10 is critical for its signaling function contributing to its interaction within these pathways. The signaling through NKG2D directly affects the activation states and responses of immune cells which are vital in immune defense.

NKG2D is implicated in cancer and autoimmune diseases. In cancer overexpression of its ligands on tumor cells makes them susceptible to NKG2D-mediated cell lysis. On the other hand aberrant expression of NKG2D ligands in autoimmune disorders can lead to inappropriate immune activation. The interaction with MICA/B proteins is significant in these conditions emphasizing its importance in both immune surveillance and potential disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
See full target information KLRK1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 17:665-77 PubMed25915125

2015

The ITIM-containing receptor LAIR1 is essential for acute myeloid leukaemia development.

Applications

Unspecified application

Species

Unspecified reactive species

Xunlei Kang,Zhigang Lu,Changhao Cui,Mi Deng,Yuqi Fan,Baijun Dong,Xin Han,Fuchun Xie,Jeffrey W Tyner,John E Coligan,Robert H Collins,Xiangshu Xiao,M James You,Cheng Cheng Zhang
View all publications
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