AB203353

Anti-NKG2D antibody

Name: Anti-NKG2D antibody (ab203353) | Abcam
Brand: Abcam Limited
SKU: AB203353
Price: 420 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(12 Publications)

Rabbit Polyclonal NKG2D antibody. Suitable for IHC-P, Flow Cyt and reacts with Mouse, Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Rat Klrk1 aa 100-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

CD314, D12S2489E, NKG2D, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2D antibody (AB203353)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2D antibody (AB203353)

Immunohistochemical analysis of formalin-fixed, paraffin embedded Human nasopharyngeal carcinoma tissue labeling NKG2D with ab203353 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Flow Cytometry - Anti-NKG2D antibody (AB203353)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2D antibody (AB203353)

Mouse thymus cells probed with ab203353 (green) at 1 : 100 dilution for 30 minutes compared to control cells (blue) and isotype control (orange)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2D antibody (AB203353)

ab203353 staining NKG2D in mouse lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 20% serum for 20 minutes at 37°C; antigen retrieval was by heat mediation in a citrate buffer for 15 minutes. Samples were incubated with primary antibody (1/400 in blocking buffer) overnight at 4°C, followed by a conjugated secondary and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, Flow Cyt

applications

Immunogen

Synthetic Peptide within Rat Klrk1 aa 100-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O70215

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "(or 1/50 -1/200 using a fluorescent secondary antibody). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "(or 1/50 -1/200 using a fluorescent secondary antibody). Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKG2D also known as natural killer group 2 member D is a type-2 transmembrane receptor with a mass of approximately 42 kDa. Expressed primarily on natural killer (NK) cells CD8+ T cells subsets of CD4+ T cells and some macrophages it recognizes stress-induced ligands presented on target cells. This protein plays an important role in the immune system's recognition and elimination of infected or transformed cells by binding to its ligands which include MHC class I chain-related proteins MICA/B and UL16-binding proteins.

Biological function summary

NKG2D acts as an activating receptor on the cell surface that initiates immune responses leading to cytotoxicity against target cells. It functions as part of a complex involving the adaptor protein DAP10 which transduces activating signals to promote cellular immune responses. This interaction amplifies the activation of immune cells and contributes to the host's defense against tumors and virally infected cells.

Pathways

NKG2D plays a significant role in immune surveillance and signaling pathways. It actively involves in the natural killer (NK) cell-mediated cytotoxicity pathway and the adaptive immune response pathway. The interaction with proteins such as DAP10 is critical for its signaling function contributing to its interaction within these pathways. The signaling through NKG2D directly affects the activation states and responses of immune cells which are vital in immune defense.

NKG2D is implicated in cancer and autoimmune diseases. In cancer overexpression of its ligands on tumor cells makes them susceptible to NKG2D-mediated cell lysis. On the other hand aberrant expression of NKG2D ligands in autoimmune disorders can lead to inappropriate immune activation. The interaction with MICA/B proteins is significant in these conditions emphasizing its importance in both immune surveillance and potential disease pathogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.

See full target information KLRK1

style

left-column

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39656086

2024

Tissue-resident natural killer cells support survival in pancreatic cancer through promotion of cDC1-CD8 T activity.

Applications

Unspecified application

Species

Unspecified reactive species

Simei Go,Constantinos Demetriou,Giampiero Valenzano,Sophie Hughes,Simone Lanfredini,Helen Ferry,Edward Arbe-Barnes,Shivan Sivakumar,Rachel Bashford-Rogers,Mark R Middleton,Somnath Mukherjee,Jennifer Morton,Keaton Jones,Eric O Neill

NPJ vaccines 9:95 PubMed38821980

2024

Neoantigen-augmented iPSC cancer vaccine combined with radiotherapy promotes antitumor immunity in poorly immunogenic cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Chih-Yang Huang,William Tzu-Liang Chen,Jia-Yi Chen,Chien-Yueh Lee,Chia-Hsin Wu,Chia-Ying Lai,Pei-Chen Yang,Ji-An Liang,An-Cheng Shiau,K S Clifford Chao,Tao-Wei Ke

Experimental & molecular medicine 56:192-209 PubMed38200153

2024

PRKCSH contributes to TNFSF resistance by extending IGF1R half-life and activation in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gu-Choul Shin,Hyeong Min Lee,Nayeon Kim,Sang-Uk Seo,Kwang Pyo Kim,Kyun-Hwan Kim

Yonsei medical journal 62:288-297 PubMed33779082

2021

Clinical Impact of Natural Killer Group 2D Receptor Expression and That of Its Ligand in Ovarian Carcinomas: A Retrospective Study.

Applications

Unspecified application

Species

Unspecified reactive species

Gee Hoon Lee,Hee Jung An,Tae Hoen Kim,Gwangil Kim,Kyung Soon Park,Hyun Park,Tae Ho Lee,Ah Young Kwon

Journal of cellular and molecular medicine 25:4596-4607 PubMed33733587

2021

CDX2 enhances natural killer cell-mediated immunotherapy against head and neck squamous cell carcinoma through up-regulating CXCL14.

Applications

Unspecified application

Species

Unspecified reactive species

Haitao Wang,Shanji Nan,Ying Wang,Chengbi Xu

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 135:111180 PubMed33433354

2021

The protective effect of 1-methyltryptophan isomers in renal ischemia-reperfusion injury is not exclusively dependent on indolamine 2,3-dioxygenase inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Diana Čepcová,Ido P Kema,Maria Sandovici,Leo E Deelman,Katarína Šišková,Ján Klimas,Peter Vavrinec,Diana Vavrincová-Yaghi

Scientific reports 10:20606 PubMed33244094

2020

Effects of obesity on NK cells in a mouse model of postmenopausal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Spielmann,Laura Mattheis,Juliane-Susanne Jung,Henrik Rauße,Markus Glaß,Ina Bähr,Dagmar Quandt,Jana Oswald,Heike Kielstein

Proceedings of the National Academy of Sciences of 117:29823-29831 PubMed33168738

2020

Excess of the NF-ĸB p50 subunit generated by the ubiquitin ligase KPC1 suppresses tumors via PD-L1- and chemokines-mediated mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Yelena Kravtsova-Ivantsiv,Gilad Goldhirsh,Alexandra Ivantsiv,Ofer Ben Itzhak,Yong Tae Kwon,Eli Pikarsky,Aaron Ciechanover

European archives of psychiatry and clinical neuroscience : PubMed31875248

2019

Splenic NKG2D confers resilience versus susceptibility in mice after chronic social defeat stress: beneficial effects of (R)-ketamine.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Zhang,Akemi Sakamoto,Lijia Chang,Youge Qu,Siming Wang,Yaoyu Pu,Yunfei Tan,Xingming Wang,Yuko Fujita,Tamaki Ishima,Masahiko Hatano,Kenji Hashimoto

Journal of immunology research 2019:2075803 PubMed30723748

2019

Sjögren's Syndrome: Concerted Triggering of Sicca Conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Zygmunt Mackiewicz,Justyna Mażul,Ieva Narkevičiūtė,Irena Dumalakienė,Irena Butrimienė,Rita Vilienė,Indrė Stankevičienė,Diana Mieliauskaitė

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NKG2D antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2D antibody (AB203353)

Flow Cytometry - Anti-NKG2D antibody (AB203353)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKG2D antibody (AB203353)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise