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AB36136

Anti-NKG2D antibody

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(5 Publications)

Goat Polyclonal NKG2D antibody. Suitable for WB, Flow Cyt and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD314, D12S2489E, NKG2D, KLRK1, NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor

2 Images
Flow Cytometry - Anti-NKG2D antibody (AB36136)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKG2D antibody (AB36136)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Western blot - Anti-NKG2D antibody (AB36136)
  • WB

Supplier Data

Western blot - Anti-NKG2D antibody (AB36136)

Lysates in RIPA buffer.

Detected by chemiluminescence.

All lanes:

Western blot - Anti-NKG2D antibody (ab36136) at 0.5 µg/mL

Lane 1:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 35 µg

Lane 2:

MOLT-4 whole cell lysate plus peptide at 35 µg

Predicted band size: 25 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "10 µg/mL", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKG2D also known as natural killer group 2 member D is a type-2 transmembrane receptor with a mass of approximately 42 kDa. Expressed primarily on natural killer (NK) cells CD8+ T cells subsets of CD4+ T cells and some macrophages it recognizes stress-induced ligands presented on target cells. This protein plays an important role in the immune system's recognition and elimination of infected or transformed cells by binding to its ligands which include MHC class I chain-related proteins MICA/B and UL16-binding proteins.
Biological function summary

NKG2D acts as an activating receptor on the cell surface that initiates immune responses leading to cytotoxicity against target cells. It functions as part of a complex involving the adaptor protein DAP10 which transduces activating signals to promote cellular immune responses. This interaction amplifies the activation of immune cells and contributes to the host's defense against tumors and virally infected cells.

Pathways

NKG2D plays a significant role in immune surveillance and signaling pathways. It actively involves in the natural killer (NK) cell-mediated cytotoxicity pathway and the adaptive immune response pathway. The interaction with proteins such as DAP10 is critical for its signaling function contributing to its interaction within these pathways. The signaling through NKG2D directly affects the activation states and responses of immune cells which are vital in immune defense.

NKG2D is implicated in cancer and autoimmune diseases. In cancer overexpression of its ligands on tumor cells makes them susceptible to NKG2D-mediated cell lysis. On the other hand aberrant expression of NKG2D ligands in autoimmune disorders can lead to inappropriate immune activation. The interaction with MICA/B proteins is significant in these conditions emphasizing its importance in both immune surveillance and potential disease pathogenesis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
See full target information KLRK1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:294 PubMed39972413

2025

The role of MUC16 in tumor biology and tumor immunology in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Na Yang,Xi Zhou,Yangmei Gong,Zhizhi Deng

Frontiers in immunology 14:1250920 PubMed38077388

2023

Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqing Wang,Xinyue Ma,Xinyu Zhang,Dizhi Jiang,Hongyuan Mao,Zerun Li,Yu Tian,Bo Cheng

Cell stem cell 23:181-192.e5 PubMed30082067

2018

Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,David L Hermanson,Branden S Moriarity,Dan S Kaufman

Oncology letters 15:747-754 PubMed29391893

2018

Expression levels of UL16 binding protein 1 and natural killer group 2 member D affect overall survival in patients with gastric cancer following gastrectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Ryoji Kamei,Kiyoshi Yoshimura,Shigefumi Yoshino,Moeko Inoue,Tetsuhiko Asao,Masanori Fuse,Satoshi Wada,Atsuo Kuramasu,Tomoko Furuya-Kondo,Atsunori Oga,Norio Iizuka,Nobuaki Suzuki,Noriko Maeda,Yusaku Watanabe,Satoshi Matsukuma,Michihisa Iida,Shigeru Takeda,Tomio Ueno,Noboru Yamamoto,Takeo Fukagawa,Hitoshi Katai,Hiroki Sasaki,Shoichi Hazama,Masaaki Oka,Hiroaki Nagano

Blood 118:3019-27 PubMed21816829

2011

Complex regulation of human NKG2D-DAP10 cell surface expression: opposing roles of the γc cytokines and TGF-β1.

Applications

WB

Species

Human

Yuk Pheel Park,Seung-Chul Choi,Patricia Kiesler,Aleksandra Gil-Krzewska,Francisco Borrego,Jennifer Weck,Konrad Krzewski,John E Coligan
View all publications
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