Anti-NKG2D antibody [EPR24072-342]

4 product images

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  Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (ab302907)

  Multichannel (Multiplex) immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of human tonsil tissue labeling NKG2D, NKG2a, and CD8 alpha.
  
  Panel A: merged staining of anti-CD8 alpha (red; Opal™690), anti-NKG2A (gray; Opal™520) and anti-NKG2D (green; Opal™570) on human tonsil.
  
  Panel B: anti-NKG2D (ab302907 at 1:2000 dilution (0.258 μg/ml)) signal on NK cells and T cell subsets.
  
  Panel C: anti-NKG2A (Anti-NKG2A antibody [EPR23737-127] ab260035 at 1:2000 dilution (0.283 μg/ml)) signal on NK cells.
  
  Panel D: anti-CD8 alpha (Anti-CD8 alpha antibody [SP239] ab178089 at 1:100 dilution (0.83 μg/ml)) signal on T cell subsets.
  
  Opal Polymer HRP Ms + Rb was used as secondary antibody.
  
  The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [SP239] ab178089, Anti-NKG2A antibody [EPR23737-127] ab260035, and ab302907 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstain was DAPI.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (ab302907)

  Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NKG2D with ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Negative control: no staining on human cardiac muscle. The section was incubated with ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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  Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (ab302907)

  Immunohistochemical analysis of paraffin-embedded Human NK/T cell lymp tissue labeling NKG2D with ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human NK/T cell lymphoma. The section was incubated with ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• 

  Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] (ab302907)

  Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NKG2D with ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human tonsil. The section was incubated with ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins