Rabbit Recombinant Monoclonal NKG2D antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Human samples.
Images
![Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--multiplex-immunohistochemistry-img409299.png/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908)")
![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401349.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908)")
![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401348.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908)")
![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401347.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (AB302908)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | mIHC | ICC/IF | Flow Cyt | IP | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
Alternative names
NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, NKG2D, D12S2489E, KLRK1
Recommended products
Rabbit Recombinant Monoclonal NKG2D antibody. Carrier free. Suitable for IHC-P, mIHC and reacts with Human samples.
Alternative names
NKG2-D type II integral membrane protein, Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, NKG2D, D12S2489E, KLRK1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR24072-342
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
NKG2D also known as natural killer group 2 member D is a type-2 transmembrane receptor with a mass of approximately 42 kDa. Expressed primarily on natural killer (NK) cells CD8+ T cells subsets of CD4+ T cells and some macrophages it recognizes stress-induced ligands presented on target cells. This protein plays an important role in the immune system's recognition and elimination of infected or transformed cells by binding to its ligands which include MHC class I chain-related proteins MICA/B and UL16-binding proteins.
- Biological function summary
NKG2D acts as an activating receptor on the cell surface that initiates immune responses leading to cytotoxicity against target cells. It functions as part of a complex involving the adaptor protein DAP10 which transduces activating signals to promote cellular immune responses. This interaction amplifies the activation of immune cells and contributes to the host's defense against tumors and virally infected cells.
- Pathways
NKG2D plays a significant role in immune surveillance and signaling pathways. It actively involves in the natural killer (NK) cell-mediated cytotoxicity pathway and the adaptive immune response pathway. The interaction with proteins such as DAP10 is critical for its signaling function contributing to its interaction within these pathways. The signaling through NKG2D directly affects the activation states and responses of immune cells which are vital in immune defense.
- Associated diseases and disorders
NKG2D is implicated in cancer and autoimmune diseases. In cancer overexpression of its ligands on tumor cells makes them susceptible to NKG2D-mediated cell lysis. On the other hand aberrant expression of NKG2D ligands in autoimmune disorders can lead to inappropriate immune activation. The interaction with MICA/B proteins is significant in these conditions emphasizing its importance in both immune surveillance and potential disease pathogenesis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--multiplex-immunohistochemistry-img409299.png "Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)")
Multiplex immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)
This data was developed using Anti-NKG2D antibody [EPR24072-342] ab302907, the same antibody clone in a different buffer formulation.
Multichannel (Multiplex) immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections of human tonsil tissue labeling NKG2D, NKG2a, and CD8 alpha.
Panel A: merged staining of anti-CD8 alpha (red; Opal™690), anti-NKG2A (gray; Opal™520) and anti-NKG2D (green; Opal™570) on human tonsil.
Panel B: anti-NKG2D (Anti-NKG2D antibody [EPR24072-342] ab302907 at 1:2000 dilution (0.258 μg/ml)) signal on NK cells and T cell subsets.
Panel C: anti-NKG2A (Anti-NKG2A antibody [EPR23737-127] ab260035 at 1:2000 dilution (0.283 μg/ml)) signal on NK cells.
Panel D: anti-CD8 alpha (Anti-CD8 alpha antibody [SP239] ab178089 at 1:100 dilution (0.83 μg/ml)) signal on T cell subsets.
Opal Polymer HRP Ms + Rb was used as secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-CD8 alpha antibody [SP239] ab178089, Anti-NKG2A antibody [EPR23737-127] ab260035, and Anti-NKG2D antibody [EPR24072-342] ab302907 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear counterstain was DAPI.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401349.jpg "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)")
Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)
This data was developed using Anti-NKG2D antibody [EPR24072-342] ab302907, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling NKG2D with Anti-NKG2D antibody [EPR24072-342] ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Negative control: no staining on human cardiac muscle. The section was incubated with Anti-NKG2D antibody [EPR24072-342] ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401348.jpg "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)")
Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)
This data was developed using Anti-NKG2D antibody [EPR24072-342] ab302907, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human NK/T cell lymp tissue labeling NKG2D with Anti-NKG2D antibody [EPR24072-342] ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human NK/T cell lymphoma. The section was incubated with Anti-NKG2D antibody [EPR24072-342] ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
![Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908), expandable thumbnail](https://content.abcam.com/products/images/nkg2d-antibody-epr24072-342-bsa-and-azide-free-ab302908--immunohistochemistry-img401347.jpg "Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)")
Immunohistochemistry - Anti-NKG2D antibody [EPR24072-342] - BSA and Azide free (ab302908)
This data was developed using Anti-NKG2D antibody [EPR24072-342] ab302907, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling NKG2D with Anti-NKG2D antibody [EPR24072-342] ab302907 at 1/2000 (0.258 ug/ml) followed by a LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Positive staining on human tonsil. The section was incubated with Anti-NKG2D antibody [EPR24072-342] ab302907 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond Polymer Refine Detection) was used at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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