AB186425

Anti-NKp30 antibody [EPR14509]

Name: Anti-NKp30 antibody [EPR14509] (ab186425) | Abcam
Brand: Abcam Limited
SKU: AB186425
Price: 335 USD
Availability: InStock
Rating: 5 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(3 Publications)

Rabbit Recombinant Monoclonal NKp30 antibody. Suitable for IP, WB and reacts with Human, Rat samples. Cited in 3 publications.

View Alternative Names

CD337, 1C7, LY117, NCR3, Natural cytotoxicity triggering receptor 3, Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30

3 Images

Supplier Data

Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (AB186425)

Western blot analysis of NK-p30 in K562 cell lysate immunoprecipitated using ab186425 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of K562 lysate. Anti-Rabbit IgG (HRP) specific to the non-reduced form of IgG was used as a secondary antibody at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (ab186425)

Predicted band size: 22 kDa

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Supplier Data

Western blot - Anti-NKp30 antibody [EPR14509] (AB186425)

All lanes:

Western blot - Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution

All lanes:

K562 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Supplier Data

Western blot - Anti-NKp30 antibody [EPR14509] (AB186425)

All lanes:

Western blot - Anti-NKp30 antibody [EPR14509] (ab186425) at 1/1000 dilution

Lane 1:

THP1 cell lysate at 10 µg

Lane 2:

NCI-H460 lysate at 10 µg

Lane 3:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 22 kDa

Observed band size: 22 kDa

false

Carrier free

Anti-NKp30 antibody [EPR14509] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14509

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKp30 also known as NCR3 (Natural Cytotoxicity Receptor 3) is a type I transmembrane protein that functions as an activating receptor on natural killer (NK) cells. It is part of the natural cytotoxicity receptor family and is encoded by the NCR3 gene. NKp30 is characterized by its structure which includes a single immunoglobulin-like domain. This protein weighs approximately 30 kDa. NKp30 is expressed mainly on resting and activated NK cells as well as IL-15-stimulated NK cells found in humans.

Biological function summary

NKp30 plays a significant role in immune response by recognizing and binding to ligands on tumor cells and virus-infected cells facilitating their elimination. It forms part of a larger immunological complex that includes DNAM-1 and 2B4 other activating receptors that work in concert to enhance the natural killing ability of NK cells. NKp30 triggers the release of cytotoxic granules and cytokines like IFN-γ leading to an enhanced cytotoxic response against target cells.

Pathways

NKp30 participates heavily in immune regulation especially in NK cell-mediated cytotoxicity. This protein is involved in pathways such as the NK cell-mediated antibody-dependent cellular cytotoxicity (ADCC) and has strong interaction with signaling proteins like CD3ζ and DAP12 which transduce activating signals. Through these pathways NKp30 influences the immune surveillance capability of NK cells modulating immune responses and maintaining cellular homeostasis.

NKp30 has been implicated in cancer progression and immune evasion notably in melanoma and gastrointestinal stromal tumors (GIST). Altered expression of NKp30 can result in reduced immune recognition and elimination of these tumor cells. The protein's interaction with B7-H6 a ligand often overexpressed on cancer cells illustrates its importance in tumor immunity. NKp30's function or dysfunction makes it a relevant target for therapeutic interventions aimed at restoring immune competence against cancerous cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell membrane receptor of natural killer/NK cells that is activated by binding of extracellular ligands including BAG6 and NCR3LG1. Stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It controls, for instance, NK cells cytotoxicity against tumor cells. Engagement of NCR3 by BAG6 also promotes myeloid dendritic cells (DC) maturation, both through killing DCs that did not acquire a mature phenotype, and inducing the release by NK cells of TNFA and IFNG which promote DC maturation.

See full target information NCR3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 39:633-646 PubMed37933909

2023

WTAP enhances the instability of SYTL1 mRNA caused by YTHDF2 in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiansong Wang,Jianjun Luo,Xuecheng Wu,Zhuo Li

Molecular and cellular biochemistry 458:159-169 PubMed31020492

2019

Biological effects of cyclosporin A on CD3CD161 and CD3CD161 lymphocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Aravinthan Adithan,Judith Sharmila John Peter,Mohammad Amjad Hossain,Chang-Won Kang,Bumseok Kim,Nam Soo Kim,Ki-Chul Hwang,Jong-Hoon Kim

International journal of molecular sciences 18: PubMed29137183

2017

Lactobacillus plantarum Enhanced IL-22 Production in Natural Killer (NK) Cells That Protect the Integrity of Intestinal Epithelial Cell Barrier Damaged by Enterotoxigenic Escherichia coli.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqin Qiu,Zongyong Jiang,Shenglan Hu,Li Wang,Xianyong Ma,Xuefen Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-NKp30 antibody [EPR14509]

Immunoprecipitation - Anti-NKp30 antibody [EPR14509] (AB186425)

Western blot - Anti-NKp30 antibody [EPR14509] (AB186425)

Western blot - Anti-NKp30 antibody [EPR14509] (AB186425)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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