Anti-NKR-P1C antibody [EPR22990-31]

9 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling NKR-P1C with ab289542 at 1/100 (5.26 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse spleen. The section was incubated with ab289542 at 4°C overnight. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab289542. Then stained with anti-CD49b conjugated to PE. Gated on viable cells.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling NKR-P1C with ab289542 at 1/100 (5.26 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on lympho node of mouse colon. The section was incubated with ab289542 at 4°C overnight. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

  Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0)

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  Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with ab289542, and then stained with anti-CD19 conjugated to PE-Cy7 or anti-CD11b conjugated to BV510. Gated on viable cells. Negative expression on B cells (Left) and Myeloid cells (Right) were observed.

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  Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug). Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with ab289542, and then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells. NK(NKR-P1C+) and NKT(CD3+NKR-P1C+) population were observed by CD3 co-staining.

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  Immunohistochemistry (Frozen sections) - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling NKR-P1C with ab289542 at 1/100 (5.26 μg/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining in mouse spleen is observed. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling NKR-P1C with ab289542 at 1/50 (10.52 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing membranal staining in subsets of mouse splenocytes is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
  

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml) dilution.

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  Multiplex immunohistochemistry - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Multiplex immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse spleen tissue staining Klra8 with Anti-Klra8 antibody [EPR28281-49] ab313388 at a 1/2000 dilution ( 0.253 μg/ml), ab289542 anti-NKR-P1C used at 1/100 dilution (5.18 μg/ml).
  
  Panel A: merged staining of anti-NKR-P1C (green; Opal™520) and anti-Klra8 (red; Opal™570) on mouse spleen.
  Panel B: anti-Klra8 stained on NK cell subsets.
  Panel C: anti-NKR-P1C stained on NK cell subsets.
  
  The section was incubated in two rounds of staining: in the order of ab289542 and Anti-Klra8 antibody [EPR28281-49] ab313388 for 30 mins at room temperature.
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
  Each round was followed by a separate fluorescent tyramide signal amplification system.
  
  The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

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  This image is courtesy of an anonymous customer review

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKR-P1C antibody [EPR22990-31] (ab289542)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of paraformaldehyde-fixed mouse lung tissue staining with ab289542 at 1/100 dilution. Secondary antibody was a ready to use HRP anti-polyvalent. Samples were incubated with the primary antibody with 1% BSA in 1x PBS for 24 hours at 4°C. Blocking was done using a ready to use 12.5% Mouse on Mouse Blocking reagent for 1 hour at 22 degrees. Heat mediated antigen retrieval with Tris/EDTA pH9 performed in a steamer at approx. 99°C for 20 min. Hematoxylin was used for counterstaining.