Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labelling NKR-P1C with ab289542 at 1/100 (5.26 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on mouse spleen. The section was incubated with ab289542 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse spleen cells labelling NKR-P1C with ab289542 at 1/50 (10.52 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing membranal staining in subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labelling NKR-P1C with ab289542 at 1/100 (5.26 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on lympho node of mouse colon. The section was incubated with ab289542 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• Flow Cyt

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Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with ab289542, and then stained with anti-CD19 conjugated to PE-Cy7 or anti-CD11b conjugated to BV510. Gated on viable cells. Negative expression on B cells (Left) and Myeloid cells (Right) were observed.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab289542. Then stained with anti-CD49b conjugated to PE. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse splenocytes cells labelling NKR-P1C with ab289542 at 1/500 dilution (0.1ug). Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with ab289542, and then stained with anti-CD3 conjugated to Alexa Fluor® 647. Gated on viable cells. NK(NKR-P1C+) and NKT(CD3+NKR-P1C+) population were observed by CD3 co-staining.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-NKR-P1C antibody [EPR22990-31] - BSA and Azide free (AB289553)

This data was developed using ab289542, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen (fresh) tissue labeling NKR-P1C with ab289542 at 1/100 (5.26 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution (Green). Positive staining in mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.