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AB327133

Anti-NKX2-2 Antibody [CL11380]

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Mouse Monoclonal Nkx2.2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-2.

View Alternative Names

NKX2.2, NKX2B, NKX2-2, Homeobox protein Nkx-2.2, Homeobox protein NK-2 homolog B

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Immunohistochemical staining of human Cerebral cortex using ab327133 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in neuronal and glial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Immunohistochemistry analysis in human cerebral cortex and liver tissues using ab327133 at 1 : 200–1 : 500 dilution. Corresponding NKX2-2 RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Immunohistochemical staining of human Pancreas using ab327133 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in islets of Langerhans.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Immunohistochemical staining of human Stomach using ab327133 at 1 : 200–1 : 500 dilution, showing strong nuclear positivity in enteroendocrine cells.

Western blot - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • WB

Supplier Data

Western blot - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Western blot analysis in human cell line U-138MG using ab327133 at 1 µg/ml.

All lanes:

Western blot - Anti-NKX2-2 Antibody [CL11380] (ab327133)

All lanes:

Human cell line U-138MG

Predicted band size: 30.13 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NKX2-2 Antibody [CL11380] (AB327133)

Immunohistochemical staining of human Liver using ab327133 at 1 : 200–1 : 500 dilution, showing no positivity in hepatocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL11380

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human NKX2-2.

O95096

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity).
See full target information NKX2-2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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