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AB244270

Anti-Nkx2.2 antibody

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(1 Publication)

Rabbit Polyclonal Nkx2.2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-2 aa 1-150.

View Alternative Names

NKX2.2, NKX2B, NKX2-2, Homeobox protein Nkx-2.2, Homeobox protein NK-2 homolog B

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)

Formalin-fixed, paraffin-embedded human pancreas tissue stained for Nkx2 with ab244270 at a 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)

Immunohistochemical staining of human cerebellum shows moderate nuclear positivity in cells in molecular layer.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue shows absence of immunoreactivity for Nkx2 when incubated with ab244270 at a 1/50 dilution (negative control).

Western blot - Anti-Nkx2.2 antibody (AB244270)
  • WB

Unknown

Western blot - Anti-Nkx2.2 antibody (AB244270)

All lanes:

Western blot - Anti-Nkx2.2 antibody (ab244270) at 0.4 µg/mL

All lanes:

U-138 MG (human glioblastoma cell line) cell lysate

Predicted band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody (AB244270)

Formalin-fixed, paraffin-embedded human cerebral cortex tissue stained for Nkx2 with ab244270 at a 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NKX2-2 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O95096

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.2 also known as NK2 homeobox 2 is a transcription factor weighing approximately 29 kDa. It plays a pivotal role in early neural development and is important for the differentiation of oligodendrocytes in the central nervous system. Nkx2.2 is expressed in the developing brain and spinal cord. Researchers often use Nkx2.2 immunohistochemistry (IHC) to study its spatial and temporal expression patterns during embryonic development. This protein is part of a group that includes other members such as Nkx2-1 and Nkx2-5 and it shares conserved domains with them.
Biological function summary

Nkx2.2 influences the regulation of specific genes essential for cell fate determination. It interacts with other factors in a regulatory network forming complexes that modulate gene expression necessary for the maturation of neural precursor cells. Notably Nkx2.2 is required for the development of insulin-producing pancreatic beta cells where it works in conjunction with other transcription factors like Pdx1 and Foxa2. Its ability to bind DNA and promote specific gene transcription positions it as an important regulator in the networks driving neural and pancreatic tissue specification.

Pathways

Nkx2.2 is important in the Sonic Hedgehog (Shh) signaling pathway which is instrumental for embryonic neural tube patterning. The Shh pathway directs Nkx2.2 expression in response to morphogen gradients to determine distinct neural progenitor domains. Nkx2.2 expression is closely linked with the transcription factor Olig2 which aligns with specific domains in the ventral neural tube. Moreover Nkx2.2 is involved in the Wnt signaling pathway playing a role in the coordination of cell differentiation and proliferation.

Nkx2.2 has associations with glioma and type 1 diabetes. Abnormal expression may contribute to glioma development by disrupting normal oligodendrocyte precursor cell development. Nkx2.2 dysregulation can also interfere with insulin regulation implicating it in type 1 diabetes progression especially through its interaction with Pdx1 an important player in pancreas development. Understanding Nkx2.2's role in these conditions aids in deciphering underlying mechanisms and provides potential therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity).
See full target information NKX2-2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in molecular neuroscience 16:1083850 PubMed37033374

2023

Involvement of endoplasmic reticulum stress in trigeminal ganglion corneal neuron injury in dry eye disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyu Zhang,Hongbin Lin,Fengxian Li,Kaili Wu,Shuangjian Yang,Shiyou Zhou
View all publications
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Product promise

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