AB272915

Anti-Nkx2.2 antibody

Name: Anti-Nkx2.2 antibody (ab272915) | Abcam
Brand: Abcam Limited
SKU: AB272915
Price: 530 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Polyclonal Nkx2.2 antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Mouse Nkx2-2.

View Alternative Names

NKX2.2, NKX2B, NKX2-2, Homeobox protein Nkx-2.2, Homeobox protein NK-2 homolog B

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody (AB272915)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody (AB272915)

4% PFA-fixed DIV9 rat E18 primary hippocampal neuron cells stained for Nkx2.2 using ab272915 at 1/500 dilution in ICC/IF.

Red : Beta tubulin.

Blue : DAPI.

Immunohistochemistry (Frozen sections) - Anti-Nkx2.2 antibody (AB272915)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Nkx2.2 antibody (AB272915)

Paraffin-embedded rat E13.5 brain tissue stained for Nkx2.2 using ab272915 at 1/250 dilution in immunohistochemical analysis.

Red : Beta tubulin.

Blue : DAPI.

Unknown

Western blot - Anti-Nkx2.2 antibody (AB272915)

12% SDS-PAGE.

All lanes:

Western blot - Anti-Nkx2.2 antibody (ab272915) at 1/5000 dilution

Lane 1:

Non-transfected HEK-293T cell lysate at 30 µg

Lane 2:

DDDDK-tagged Nkx2.2 transfected HEK-293T cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 30 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

WB, ICC/IF, IHC-Fr

applications

Immunogen

Recombinant Fragment Protein within Mouse Nkx2-2. The exact immunogen used to generate this antibody is proprietary information.

P42586

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/1000", "IHCFr-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.2 also known as NK2 homeobox 2 is a transcription factor weighing approximately 29 kDa. It plays a pivotal role in early neural development and is important for the differentiation of oligodendrocytes in the central nervous system. Nkx2.2 is expressed in the developing brain and spinal cord. Researchers often use Nkx2.2 immunohistochemistry (IHC) to study its spatial and temporal expression patterns during embryonic development. This protein is part of a group that includes other members such as Nkx2-1 and Nkx2-5 and it shares conserved domains with them.

Biological function summary

Nkx2.2 influences the regulation of specific genes essential for cell fate determination. It interacts with other factors in a regulatory network forming complexes that modulate gene expression necessary for the maturation of neural precursor cells. Notably Nkx2.2 is required for the development of insulin-producing pancreatic beta cells where it works in conjunction with other transcription factors like Pdx1 and Foxa2. Its ability to bind DNA and promote specific gene transcription positions it as an important regulator in the networks driving neural and pancreatic tissue specification.

Pathways

Nkx2.2 is important in the Sonic Hedgehog (Shh) signaling pathway which is instrumental for embryonic neural tube patterning. The Shh pathway directs Nkx2.2 expression in response to morphogen gradients to determine distinct neural progenitor domains. Nkx2.2 expression is closely linked with the transcription factor Olig2 which aligns with specific domains in the ventral neural tube. Moreover Nkx2.2 is involved in the Wnt signaling pathway playing a role in the coordination of cell differentiation and proliferation.

Nkx2.2 has associations with glioma and type 1 diabetes. Abnormal expression may contribute to glioma development by disrupting normal oligodendrocyte precursor cell development. Nkx2.2 dysregulation can also interfere with insulin regulation implicating it in type 1 diabetes progression especially through its interaction with Pdx1 an important player in pancreas development. Understanding Nkx2.2's role in these conditions aids in deciphering underlying mechanisms and provides potential therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity).

See full target information NKX2-2

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 30:9636897211048786 PubMed34606735

2021

Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat.

Applications

Unspecified application

Species

Unspecified reactive species

Samaneh Khazaei,Masoud Soleimani,Seyed Hossein Ahmadi Tafti,Rouhollah Mehdinavaz Aghdam,Zohreh Hojati

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nkx2.2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody (AB272915)

Immunohistochemistry (Frozen sections) - Anti-Nkx2.2 antibody (AB272915)

Western blot - Anti-Nkx2.2 antibody (AB272915)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise