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Rabbit Recombinant Monoclonal Nkx2.2 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (AB240318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (AB240318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (AB240318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (AB240318), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (AB240318), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIHC-P
Human
Expected
Tested
Mouse
Expected
Tested
Rat
Tested
Tested

Tested
Tested

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas (By similarity). May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter (By similarity).

Alternative names

NKX2.2, NKX2B, NKX2-2, Homeobox protein Nkx-2.2, Homeobox protein NK-2 homolog B

Recommended products

Rabbit Recombinant Monoclonal Nkx2.2 antibody. Carrier free. Suitable for ICC/IF, IHC-P and reacts with Rat, Mouse, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14638
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab240318 is the carrier-free version of Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nkx2.2 also known as NK2 homeobox 2 is a transcription factor weighing approximately 29 kDa. It plays a pivotal role in early neural development and is important for the differentiation of oligodendrocytes in the central nervous system. Nkx2.2 is expressed in the developing brain and spinal cord. Researchers often use Nkx2.2 immunohistochemistry (IHC) to study its spatial and temporal expression patterns during embryonic development. This protein is part of a group that includes other members such as Nkx2-1 and Nkx2-5 and it shares conserved domains with them.

Biological function summary

Nkx2.2 influences the regulation of specific genes essential for cell fate determination. It interacts with other factors in a regulatory network forming complexes that modulate gene expression necessary for the maturation of neural precursor cells. Notably Nkx2.2 is required for the development of insulin-producing pancreatic beta cells where it works in conjunction with other transcription factors like Pdx1 and Foxa2. Its ability to bind DNA and promote specific gene transcription positions it as an important regulator in the networks driving neural and pancreatic tissue specification.

Pathways

Nkx2.2 is important in the Sonic Hedgehog (Shh) signaling pathway which is instrumental for embryonic neural tube patterning. The Shh pathway directs Nkx2.2 expression in response to morphogen gradients to determine distinct neural progenitor domains. Nkx2.2 expression is closely linked with the transcription factor Olig2 which aligns with specific domains in the ventral neural tube. Moreover Nkx2.2 is involved in the Wnt signaling pathway playing a role in the coordination of cell differentiation and proliferation.

Associated diseases and disorders

Nkx2.2 has associations with glioma and type 1 diabetes. Abnormal expression may contribute to glioma development by disrupting normal oligodendrocyte precursor cell development. Nkx2.2 dysregulation can also interfere with insulin regulation implicating it in type 1 diabetes progression especially through its interaction with Pdx1 an important player in pancreas development. Understanding Nkx2.2's role in these conditions aids in deciphering underlying mechanisms and provides potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed C6 cells labeling Nkx2.2 with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/50, Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 and DAPI staining (blue). Negative controls also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling Nkx2.2 with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling Nkx2.2 with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Nkx2.2 with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    Immunohistochemical analysis of paraffin-embedded Human Ewing sarcoma tissue labeling Nkx2.2 with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/1000. Secondary antibody Goat anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin. Negative control also shown.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue staining with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/6000 dilution. The secondary was ready-to-use LeicaDS9800 (Bond™ Polymer Refine Detection), and the counterstain was Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on human pancreatic islets. The section was incubated with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse pancreas tissue staining with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/6000 dilution. The secondary was ready-to-use LeicaDS9800 (Bond™ Polymer Refine Detection), and the counterstain was Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on mouse pancreatic islet. The section was incubated with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.2 antibody [EPR14638] - BSA and Azide free (ab240318)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077).

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat pancreas tissue staining with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 at 1/6000 dilution. The secondary was ready-to-use LeicaDS9800 (Bond™ Polymer Refine Detection), and the counterstain was Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. Positive staining on rat pancreatic islets. The section was incubated with Anti-Nkx2.2 antibody [EPR14638] - N-terminal ab191077 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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