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AB189202

Anti-Nkx2.4 antibody

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(3 Publications)

Rabbit Polyclonal Nkx2.4 antibody. Suitable for ICC, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human NKX2-4.

View Alternative Names

NKX2D, NKX2-4, Homeobox protein Nkx-2.4, Homeobox protein NK-2 homolog D

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.4 antibody (AB189202)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.4 antibody (AB189202)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin tissue labeling Nkx2.4 with ab189202 at 5 μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.4 antibody (AB189202)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.4 antibody (AB189202)

Immunofluorescent analysis of A20 cells labeling Nkx2.4 with ab189202 at 20 μg/ml.

Immunocytochemistry - Anti-Nkx2.4 antibody (AB189202)
  • ICC

Supplier Data

Immunocytochemistry - Anti-Nkx2.4 antibody (AB189202)

Immunocytochemical analysis of A20 cells labeling Nkx2.4 with ab189202 at 2.5 μg/ml.

Western blot - Anti-Nkx2.4 antibody (AB189202)
  • WB

Supplier Data

Western blot - Anti-Nkx2.4 antibody (AB189202)

All lanes:

Western blot - Anti-Nkx2.4 antibody (ab189202) at 1 µg/mL

Lane 1:

A20 cell lysate

Lane 2:

A20 cell lysate with immunizing peptide

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC, ICC/IF

applications

Immunogen

Synthetic Peptide within Human NKX2-4. The exact immunogen used to generate this antibody is proprietary information.

Q9H2Z4

Specificity

ab189202 is predicted to not cross-react with other NK2 homeobox family members. At least two isoforms of Nkx2.4 are known to exist; this antibody will detect both isoforms.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2.5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.4 also known as a homeobox protein Nkx-2.4 is a transcription factor that influences gene expression important for developmental processes. This protein has a molecular mass of approximately 29 kDa. Nkx2.4 expression occurs mainly in the developing neural tissues and some other tissues during embryogenesis. Researchers find it critical in the patterning and differentiation of these tissues often interacting with DNA sequences to regulate specific gene expression.
Biological function summary

Nkx2.4 contributes to neural differentiation and development. It acts independently or in conjunction with other proteins as part of larger transcriptional complexes. These complexes ensure proper progression in developmental processes as Nkx2.4 guides target gene expression during early development. Its involvement is linked to controlling the formation of various neural structures demonstrating its fundamental role in embryonic development.

Pathways

Nkx2.4 is important in the formation and differentiation within the Wnt signaling and Notch pathways. It collaborates with other proteins like Sox2 and Pax6 performing specific roles that aid in defining the development of neural cells. Nkx2.4 regulates genes situating early in these pathways guiding cell-fate decisions and structural development during embryogenesis.

Nkx2.4's malfunction links to neurological disorders and developmental abnormalities. Misregulation or mutations in Nkx2.4 have associations with certain congenital conditions affecting the neural system. It also interacts with proteins such as Lhx3 in disease contexts emphasizing the need for proper Nkx2.4 function to ensure typical neural development and maintain health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable transcription factor.
See full target information NKX2-4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Regenerative biomaterials 11:rbae072 PubMed38974665

2024

Promotion of cardiac microtissue assembly within G-CSF-enriched collagen I-cardiogel hybrid hydrogel.

Applications

Unspecified application

Species

Unspecified reactive species

Hamid Khodayari,Saeed Khodayari,Malihe Rezaee,Siamak Rezaeiani,Mahmoud Alipour Choshali,Saiedeh Erfanian,Ahad Muhammadnejad,Fatemeh Nili,Yasaman Pourmehran,Reihaneh Pirjani,Sarah Rajabi,Naser Aghdami,Canan Nebigil-Désaubry,Kai Wang,Habibollah Mahmoodzadeh,Sara Pahlavan

American journal of human genetics 109:2196-2209 PubMed36459980

2022

Functional characterization of 5p15.33 risk locus in uveal melanoma reveals rs452384 as a functional variant and NKX2.4 as an allele-specific interactor.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Céline Derrien,Alexandre Houy,Olivier Ganier,Florent Dingli,Massih Ningarhari,Lenha Mobuchon,María Isabel Espejo Díaz,Damarys Loew,Nathalie Cassoux,Olivier Cussenot,Géraldine Cancel-Tassin,Raphael Margueron,Josselin Noirel,Jessica Zucman-Rossi,Manuel Rodrigues,Marc-Henri Stern

Nucleic acids research 46:2290-2307 PubMed29294103

2018

Brain-specific deletion of histone variant H2A.z results in cortical neurogenesis defects and neurodevelopmental disorder.

Applications

Unspecified application

Species

Unspecified reactive species

Tianjin Shen,Fen Ji,Yuanyuan Wang,Xuepei Lei,Dongming Zhang,Jianwei Jiao
View all publications
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