AB106923

Anti-Nkx2.5 antibody

Name: Anti-Nkx2.5 antibody (ab106923) | Abcam
Brand: Abcam Limited
SKU: AB106923
Price: 420 USD
Availability: InStock
Rating: 3 (4 reviews)

(4 Reviews)

(12 Publications)

Goat Polyclonal Nkx2.5 antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Mouse samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human NKX2-5 aa 50-150.

View Alternative Names

CSX, NKX2.5, NKX2E, NKX2-5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (AB106923)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (AB106923)

Immunohistochemical analysis of human heart tissue stained for Nkx2.5 using ab106923 at 4μg/ml. Positive cytoplasmic staining shown.

Supplier Data

Immunocytochemistry - Anti-Nkx2.5 antibody (AB106923)

Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 μg/mL followed by Alexa Fluor^® 488 secondary antibody at 2 μg/mL. The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor^® 488 secondary antibody (2 μg/mL).

Supplier Data

Western blot - Anti-Nkx2.5 antibody (AB106923)

Detected by chemiluminescence.

All lanes:

Western blot - Anti-Nkx2.5 antibody (ab106923) at 1 µg/mL

Lane 1:

Human heart lysate in RIPA buffer at 35 µg

Lane 2:

Mouse heart lysate in RIPA buffer at 35 µg

Lane 3:

Rat heart lysate in RIPA buffer at 35 µg

Observed band size: 45 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human NKX2-5 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P52952

Specificity

ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "Only suitable for human, not mouse samples." }, "Mouse": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent.", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab106923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.5 also known as NKX2-5 or CSX is a homeobox-containing transcription factor with a molecular mass of approximately 35 kDa. It is primarily expressed in the heart specifically in cardiac progenitor cells and also found in low levels in the spleen stomach and pancreas. As a transcription factor Nkx2.5 plays a significant role in regulating the expression of genes critical for cardiac development and differentiation. The protein's expression is tightly regulated during early embryogenesis and maintained in the heart throughout life.

Biological function summary

Nkx2.5 functions to direct the development of cardiac myocytes by regulating genes involved in cell specification and tissue formation. Nkx2.5 does not solely operate but interacts with other cardiac-specific transcription factors such as GATA4 and TBX5 collectively forming a transcriptional complex. This cooperative interaction is essential in orchestrating the genetic program necessary for heart morphogenesis and cellular proliferation within the developing myocardium.

Pathways

The protein Nkx2.5 significantly influences cardiogenesis and heart formation pathways. Its activity is necessary in the Wnt signaling pathway important for the initial stages of cardiac cell lineage differentiation. Additionally Nkx2.5 works in tandem with the protein MEF2C to regulate cardiac-specific genes ensuring the proper development and functioning of cardiac tissues. Both pathways highlight important regulatory networks where Nkx2.5 is an important player in heart development.

Mutations or dysregulations of Nkx2.5 can lead to congenital heart defects such as atrial septal defects and tetralogy of Fallot. These conditions often arise due to disrupted genetic signaling during heart formation. Nkx2.5 mutations have been associated with conduction system defects in conjunction with the GATA4 protein both known to be involved in maintaining normal cardiac structure and function. Understanding Nkx2.5's role in these diseases aides in developing potential therapeutic approaches targeting congenital heart abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor required for the development of the heart and the spleen (PubMed : 22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed : 22849347, PubMed : 26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed : 22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed : 29899023).

See full target information NKX2-5

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 53:45-57 PubMed40688020

2025

Stromal-platelet membrane-inspired nanoparticles (SPIN) for targeted heart repair.

Applications

Unspecified application

Species

Unspecified reactive species

Mingqian He,Yuan Li,Dashuai Zhu,Junlang Li,Meggie Cangu,Panagiotis Tasoudis,Jiazhu Xu,Thomas G Caranasos,Yi Hong,Ke Huang

STAR protocols 5:103452 PubMed39520685

2024

Protocol for quantifying murine cardiomyocyte cell division by single-cell suspension.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Swift,Alexandra L Purdy,Michaela Patterson

Cell reports methods 4:100860 PubMed39255794

2024

A broadly applicable method for quantifying cardiomyocyte cell division identifies proliferative events following myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Swift,Alexandra L Purdy,Tyler Buddell,Jerrell J Lovett,Smrithi V Chanjeevaram,Anooj Arkatkar,Caitlin C O'Meara,Michaela Patterson

American journal of physiology. Heart and circulatory physiology 327:H377-H389 PubMed38847758

2024

is sufficient but not required for cardiomyocyte cell-cycle activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaelin A Akins,Michael A Flinn,Samantha K Swift,Smrithi V Chanjeevaram,Alexandra L Purdy,Tyler Buddell,Mary E Kolell,Kaitlyn G Andresen,Samantha Paddock,Sydney L Buday,Matthew B Veldman,Caitlin C O'Meara,Michaela Patterson

Development (Cambridge, England) 150: PubMed36912240

2023

Cardiomyocyte ploidy is dynamic during postnatal development and varies across genetic backgrounds.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha K Swift,Alexandra L Purdy,Mary E Kolell,Kaitlyn G Andresen,Caitlin Lahue,Tyler Buddell,Kaelin A Akins,Christoph D Rau,Caitlin C O'Meara,Michaela Patterson

Molecular medicine reports 24: PubMed34664684

2021

Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Boshi Yu,Mengmeng Li,Shu Ping Han,Zhangbin Yu,Jingai Zhu

Nature communications 12:1412 PubMed33658506

2021

Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dashuai Zhu,Zhenhua Li,Ke Huang,Thomas G Caranasos,Joseph S Rossi,Ke Cheng

Experimental and therapeutic medicine 18:4631-4636 PubMed31798700

2019

Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Chen,Shunen Xu,Wei Li,Lirong Wu,Long Wang,Yongkang Li,Wei Zhou

Human molecular genetics 28:3137-3147 PubMed31313802

2019

A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sumantra Chatterjee,Aravinda Chakravarti

Advanced functional materials 29: PubMed32256277

2019

Platelet-Inspired Nanocells for Targeted Heart Repair After Ischemia/Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Su,Ke Huang,Hong Ma,Hongxia Liang,Phuong-Uyen Dinh,Justin Chen,Deliang Shen,Tyler A Allen,Li Qiao,Zhenhua Li,Shiqi Hu,Jhon Cores,Brianna N Frame,Ashlyn T Young,Qi Yin,Jiandong Liu,Li Qian,Thomas G Caranasos,Yevgeny Brudno,Frances S Ligler,Ke Cheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Nkx2.5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (AB106923)

Immunocytochemistry - Anti-Nkx2.5 antibody (AB106923)

Western blot - Anti-Nkx2.5 antibody (AB106923)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise