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AB97355

Anti-Nkx2.5 antibody

3

(4 Reviews)

|

(13 Publications)

Rabbit Polyclonal Nkx2.5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-5 aa 1-200.

View Alternative Names

CSX, NKX2.5, NKX2E, NKX2-5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.5 antibody (AB97355)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.5 antibody (AB97355)

Mouse ESC D3 cells stained for Nkx2.5 (green) using ab97355 at 1/200 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (AB97355)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (AB97355)

Paraffin embedded rat heart tissue stained for Nkx2.5 using ab97355 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Nkx2.5 antibody (AB97355)
  • WB

Unknown

Western blot - Anti-Nkx2.5 antibody (AB97355)

10% SDS PAGE

All lanes:

Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution

Lane 1:

H1299 whole cell lysate at 30 µg

Lane 2:

Raji whole cell lysate at 30 µg

Predicted band size: 35 kDa

false

Western blot - Anti-Nkx2.5 antibody (AB97355)
  • WB

Supplier Data

Western blot - Anti-Nkx2.5 antibody (AB97355)

12% SDS-PAGE

All lanes:

Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution

Lane 1:

HEK-293T whole cell extract at 30 µg

Lane 2:

A549 whole cell extract at 30 µg

Lane 3:

MCF-7 whole cell extract at 30 µg

Predicted band size: 35 kDa

false

Western blot - Anti-Nkx2.5 antibody (AB97355)
  • WB

Supplier Data

Western blot - Anti-Nkx2.5 antibody (AB97355)

12% SDS-PAGE

All lanes:

Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution

Lane 1:

Mouse heart tissue extract at 50 µg

Lane 2:

Mouse spleen tissue extract at 50 µg

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human NKX2-5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

P52952

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx2.5 also known as NKX2-5 or CSX is a homeobox-containing transcription factor with a molecular mass of approximately 35 kDa. It is primarily expressed in the heart specifically in cardiac progenitor cells and also found in low levels in the spleen stomach and pancreas. As a transcription factor Nkx2.5 plays a significant role in regulating the expression of genes critical for cardiac development and differentiation. The protein's expression is tightly regulated during early embryogenesis and maintained in the heart throughout life.
Biological function summary

Nkx2.5 functions to direct the development of cardiac myocytes by regulating genes involved in cell specification and tissue formation. Nkx2.5 does not solely operate but interacts with other cardiac-specific transcription factors such as GATA4 and TBX5 collectively forming a transcriptional complex. This cooperative interaction is essential in orchestrating the genetic program necessary for heart morphogenesis and cellular proliferation within the developing myocardium.

Pathways

The protein Nkx2.5 significantly influences cardiogenesis and heart formation pathways. Its activity is necessary in the Wnt signaling pathway important for the initial stages of cardiac cell lineage differentiation. Additionally Nkx2.5 works in tandem with the protein MEF2C to regulate cardiac-specific genes ensuring the proper development and functioning of cardiac tissues. Both pathways highlight important regulatory networks where Nkx2.5 is an important player in heart development.

Mutations or dysregulations of Nkx2.5 can lead to congenital heart defects such as atrial septal defects and tetralogy of Fallot. These conditions often arise due to disrupted genetic signaling during heart formation. Nkx2.5 mutations have been associated with conduction system defects in conjunction with the GATA4 protein both known to be involved in maintaining normal cardiac structure and function. Understanding Nkx2.5's role in these diseases aides in developing potential therapeutic approaches targeting congenital heart abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor required for the development of the heart and the spleen (PubMed : 22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed : 22849347, PubMed : 26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed : 22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed : 29899023).
See full target information NKX2-5
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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 69: PubMed40984793

2025

miR-199a-5p inhibited HIF-1α to suppress the proliferation, migration, and differentiation of cardiac stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sha Li,Cuigai Zhang,Yanping Liu,Cuiqiao Meng,Yang Xie,Shuren Li

Nature cell biology 25:1033-1046 PubMed37264180

2023

Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Han,Dayang Huang,Shiyong Wu,Sheng Liu,Cheng Wang,Yi Sheng,Xiongbin Lu,Hal E Broxmeyer,Jun Wan,Lei Yang

Turkish journal of biology = Turk biyoloji dergisi 47:170-185 PubMed37529419

2023

Functional enhancement of acute infracted heart by coinjection of autologous adipose-derived stem cells with matrigel.

Applications

Unspecified application

Species

Unspecified reactive species

Bao-Zhu Wang,Meng-Meng Wang,Yan Li,Mei-Hua Shao,Dan Zhang,Shi-Qi Yan,Xiang Ma,Yi-Tong Ma

Nature communications 14:1796 PubMed37002228

2023

Endothelial deletion of PTBP1 disrupts ventricular chamber development.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Liu,Ran Duan,Xiaoyu He,Jincu Qi,Tianming Xing,Yahan Wu,Liping Zhou,Lingling Wang,Yujing Shao,Fulei Zhang,Huixing Zhou,Xingdong Gu,Bowen Lin,Yuanyuan Liu,Yan Wang,Yi Liu,Li Li,Dandan Liang,Yi-Han Chen

Cell 185:3290-3306.e25 PubMed35988542

2022

Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.

Applications

Unspecified application

Species

Unspecified reactive species

Shadi Tarazi,Alejandro Aguilera-Castrejon,Carine Joubran,Nadir Ghanem,Shahd Ashouokhi,Francesco Roncato,Emilie Wildschutz,Montaser Haddad,Bernardo Oldak,Elidet Gomez-Cesar,Nir Livnat,Sergey Viukov,Dmitry Lokshtanov,Segev Naveh-Tassa,Max Rose,Suhair Hanna,Calanit Raanan,Ori Brenner,Merav Kedmi,Hadas Keren-Shaul,Tsvee Lapidot,Itay Maza,Noa Novershtern,Jacob H Hanna

Cell death discovery 8:149 PubMed35365624

2022

The impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress.

Applications

Unspecified application

Species

Unspecified reactive species

Isotta Chimenti,Vittorio Picchio,Francesca Pagano,Leonardo Schirone,Sonia Schiavon,Luca D'Ambrosio,Valentina Valenti,Maurizio Forte,Flavio di Nonno,Speranza Rubattu,Mariangela Peruzzi,Francesco Versaci,Ernesto Greco,Antonella Calogero,Elena De Falco,Giacomo Frati,Sebastiano Sciarretta

Stem cell research 61:102757 PubMed35339881

2022

Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13.

Applications

Unspecified application

Species

Unspecified reactive species

Carina Maranga,Carolina Pereira,Ana Cláudia Raposo,Adriana Vieira,Sofia Duarte,Evguenia P Bekman,Inês Milagre,Simão Teixeira da Rocha

Scientific reports 11:3588 PubMed33574455

2021

Smad4 regulates the nuclear translocation of Nkx2-5 in cardiac differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Wenyu Hu,Anqi Dong,Kohei Karasaki,Shota Sogabe,Daiki Okamoto,Masato Saigo,Mari Ishida,Masao Yoshizumi,Hiroki Kokubo

Journal of biomedical materials research. Part B, Applied biomaterials 108:3302-3310 PubMed32524765

2020

Decellularized muscle-derived hydrogels support in vitro cardiac microtissue fabrication.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Rajabi,Nasser Aghdami,Fahimeh Varzideh,Melika Parchehbaf-Kashani,Fatemeh Nobakht Lahrood

Biomaterials 253:120060 PubMed32450407

2020

Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface.

Applications

Unspecified application

Species

Unspecified reactive species

Tzu-Cheng Sung,Huan Chiao Su,Qing-Dong Ling,S Suresh Kumar,Yung Chang,Shih-Tien Hsu,Akon Higuchi
View all publications
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