Rabbit Polyclonal Nkx2.5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-5 aa 1-200.
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- Nature cell biology 25:1033-10462023Lipid droplet-associated lncRNA LIPTER preserves cardiac lipid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLei Han,Dayang Huang,Shiyong Wu,Sheng Liu,Cheng Wang,Yi Sheng,Xiongbin Lu,Hal E Broxmeyer,Jun Wan,Lei YangPubMed 37264180
- Turkish journal of biology = Turk biyoloji dergisi 47:170-1852023Functional enhancement of acute infracted heart by coinjection of autologous adipose-derived stem cells with matrigel.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBao-Zhu Wang,Meng-Meng Wang,Yan Li,Mei-Hua Shao,Dan Zhang,Shi-Qi Yan,Xiang Ma,Yi-Tong MaPubMed 37529419
- Cell 185:3290-3306.e252022Post-gastrulation synthetic embryos generated ex utero from mouse naive ESCs.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShadi Tarazi,Alejandro Aguilera-Castrejon,Carine Joubran,Nadir Ghanem,Shahd Ashouokhi,Francesco Roncato,Emilie Wildschutz,Montaser Haddad,Bernardo Oldak,Elidet Gomez-Cesar,Nir Livnat,Sergey Viukov,Dmitry Lokshtanov,Segev Naveh-Tassa,Max Rose,Suhair Hanna,Calanit Raanan,Ori Brenner,Merav Kedmi,Hadas Keren-Shaul,Tsvee Lapidot,Itay Maza,Noa Novershtern,Jacob H HannaPubMed 35988542
- Cell death discovery 8:1492022The impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIsotta Chimenti,Vittorio Picchio,Francesca Pagano,Leonardo Schirone,Sonia Schiavon,Luca D'Ambrosio,Valentina Valenti,Maurizio Forte,Flavio di Nonno,Speranza Rubattu,Mariangela Peruzzi,Francesco Versaci,Ernesto Greco,Antonella Calogero,Elena De Falco,Giacomo Frati,Sebastiano SciarrettaPubMed 35365624
- Stem cell research 61:1027572022Generation and characterization of induced pluripotent stem cell line (IBBISTi004-A) from an Angelman syndrome patient carrying a class II deletion of the maternal chromosome 15q11.2-q13.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarina Maranga,Carolina Pereira,Ana Cláudia Raposo,Adriana Vieira,Sofia Duarte,Evguenia P Bekman,Inês Milagre,Simão Teixeira da RochaPubMed 35339881
- Scientific reports 11:35882021Smad4 regulates the nuclear translocation of Nkx2-5 in cardiac differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenyu Hu,Anqi Dong,Kohei Karasaki,Shota Sogabe,Daiki Okamoto,Masato Saigo,Mari Ishida,Masao Yoshizumi,Hiroki KokuboPubMed 33574455
- Biomaterials 253:1200602020Efficient differentiation of human pluripotent stem cells into cardiomyocytes on cell sorting thermoresponsive surface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTzu-Cheng Sung et. al.PubMed 32450407
- Molecular medicine reports 14:637-422016Shox2 influences mesenchymal stem cell fate in a co-culture model in vitro.Applications:WBReactive species:Unspecified reactive speciesYuanyuan Feng,Pan Yang,Shouming Luo,Zhihui Zhang,Huakang Li,Ping Zhu,Zhiyuan SongPubMed 27222368
- Cell cycle (Georgetown, Tex.) 15:1248-562016Cadaveric cardiosphere-derived cells can maintain regenerative capacity and improve the heart function of cardiomyopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Sun et. al.PubMed 27058215
- PloS one 6:e199642011Isolation and characterization of porcine amniotic fluid-derived multipotent stem cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human NKX2-5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P52952
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Mouse | Tested | Expected | Tested |
| Rat | Expected | Tested | Expected |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 - 1/1000 | Notes - |
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor required for the development of the heart and the spleen (PubMed:22560297). During heart development, acts as a transcriptional activator of NPPA/ANF in cooperation with GATA4 (By similarity). May cooperate with TBX2 to negatively modulate expression of NPPA/ANF in the atrioventricular canal (By similarity). Binds to the core DNA motif of NPPA promoter (PubMed:22849347, PubMed:26926761). Together with PBX1, required for spleen development through a mechanism that involves CDKN2B repression (PubMed:22560297). Positively regulates transcription of genes such as COL3A1 and MMP2, resulting in increased pulmonary endothelial fibrosis in response to hypoxia (PubMed:29899023).
Alternative names
CSX, NKX2.5, NKX2E, NKX2-5, Homeobox protein Nkx-2.5, Cardiac-specific homeobox, Homeobox protein CSX, Homeobox protein NK-2 homolog E
Recommended products
Rabbit Polyclonal Nkx2.5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 10 publications. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-5 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human NKX2-5 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P52952
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nkx2.5 also known as NKX2-5 or CSX is a homeobox-containing transcription factor with a molecular mass of approximately 35 kDa. It is primarily expressed in the heart specifically in cardiac progenitor cells and also found in low levels in the spleen stomach and pancreas. As a transcription factor Nkx2.5 plays a significant role in regulating the expression of genes critical for cardiac development and differentiation. The protein's expression is tightly regulated during early embryogenesis and maintained in the heart throughout life.
- Biological function summary
Nkx2.5 functions to direct the development of cardiac myocytes by regulating genes involved in cell specification and tissue formation. Nkx2.5 does not solely operate but interacts with other cardiac-specific transcription factors such as GATA4 and TBX5 collectively forming a transcriptional complex. This cooperative interaction is essential in orchestrating the genetic program necessary for heart morphogenesis and cellular proliferation within the developing myocardium.
- Pathways
The protein Nkx2.5 significantly influences cardiogenesis and heart formation pathways. Its activity is necessary in the Wnt signaling pathway important for the initial stages of cardiac cell lineage differentiation. Additionally Nkx2.5 works in tandem with the protein MEF2C to regulate cardiac-specific genes ensuring the proper development and functioning of cardiac tissues. Both pathways highlight important regulatory networks where Nkx2.5 is an important player in heart development.
- Associated diseases and disorders
Mutations or dysregulations of Nkx2.5 can lead to congenital heart defects such as atrial septal defects and tetralogy of Fallot. These conditions often arise due to disrupted genetic signaling during heart formation. Nkx2.5 mutations have been associated with conduction system defects in conjunction with the GATA4 protein both known to be involved in maintaining normal cardiac structure and function. Understanding Nkx2.5's role in these diseases aides in developing potential therapeutic approaches targeting congenital heart abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-Nkx2.5 antibody (ab97355)
10% SDS PAGE
All lanes: Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution
Lane 1: H1299 whole cell lysate at 30 µg
Lane 2: Raji whole cell lysate at 30 µg
Predicted band size: 35 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.5 antibody (ab97355)
Immunofluorescence analysis of Nkx2.5 in paraformaldehyde fixed HeLa, using ab97355 at a 1/200 dilution.
Lower image was merged with DNA probe.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx2.5 antibody (ab97355)
Paraffin embedded rat heart tissue stained for Nkx2.5 using ab97355 at 1/500 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-Nkx2.5 antibody (ab97355)
Mouse ESC D3 cells stained for Nkx2.5 (green) using ab97355 at 1/200 dilution in ICC/IF.
Western blot - Anti-Nkx2.5 antibody (ab97355)
12% SDS-PAGE
All lanes: Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution
Lane 1: HEK-293T whole cell extract at 30 µg
Lane 2: A549 whole cell extract at 30 µg
Lane 3: MCF-7 whole cell extract at 30 µg
Predicted band size: 35 kDa
Western blot - Anti-Nkx2.5 antibody (ab97355)
12% SDS-PAGE
All lanes: Western blot - Anti-Nkx2.5 antibody (ab97355) at 1/1000 dilution
Lane 1: Mouse heart tissue extract at 50 µg
Lane 2: Mouse spleen tissue extract at 50 µg
Predicted band size: 35 kDa
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