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AB270292

Anti-NKX2.8 antibody [NKX28/3233R] - BSA and Azide free

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Rabbit Recombinant Monoclonal NKX2.8 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NKX2-8 aa 1-150.

View Alternative Names

NKX-2.8, NKX2G, NKX2H, NKX2-8, Homeobox protein Nkx-2.8, Homeobox protein NK-2 homolog H

1 Images
Western blot - Anti-NKX2.8 antibody [NKX28/3233R] - BSA and Azide free (AB270292)
  • WB

Supplier Data

Western blot - Anti-NKX2.8 antibody [NKX28/3233R] - BSA and Azide free (AB270292)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270261).

All lanes:

Anti-NKX2.8 antibody [NKX28/3233R] (ab270261) at 2 µg/mL

Lane 1:

PC3 (Human prostate adenocarcinoma cell line) cell lysate

Lane 2:

MCF-7 (Human breast adenocarcinoma cell line) cell lysate

Predicted band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

NKX28/3233R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human NKX2-8 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O15522

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Reactivity data

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Product details

ab270292 is a carrier free version of ab270261.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Purification notes
Purified from bioreactor concentrate.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

NKX2.8 also known as NK2 homeobox 8 is a transcription factor weighing approximately 35 kDa. This protein contains a DNA-binding domain that allows it to influence gene expression. NKX2.8 acts within the nucleus and predominantly appears in tissues such as the lung and heart suggesting a role in organ-specific gene regulation. By binding to specific DNA sequences it modulates the transcription process and affects cellular functions.
Biological function summary

NKX2.8 plays a role in gene regulation during development. It is essential for proper organ morphogenesis where it helps guide tissue formation and differentiation. This protein may not necessarily form large complexes like some other transcription factors but it often interacts with different co-factors that assist its function. Such participation highlights its importance in the regulation of genes important for developmental processes.

Pathways

The NKX2.8 protein is involved in developmental pathways such as the Notch signaling pathway. In this context it impacts cellular differentiation and proliferation by interacting with proteins like Notch receptors and ligands. NKX2.8 may also have a connection to the Wnt signaling pathway affecting cell fate determination and embryonic patterning. Through these pathways NKX2.8 influences early development and organogenesis.

Studies indicate NKX2.8 involvement in congenital conditions such as congenital heart disease. Altered expression or mutations in NKX2.8 disrupt normal cardiac development. Additionally its dysregulation has an association with lung cancer where it may be linked to abnormal cell proliferation through pathways and proteins including beta-catenin. These connections emphasize the role of NKX2.8 in both development and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information NKX2-8
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