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AB250875

Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Nkx3.1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NKX3.1, NKX3A, NKX3-1, Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (AB250875)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (AB250875)

This data was developed using ab186413, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human prostate tissue labeling Nkx3.1 with ab186413 at 1/4000 dilution and ready to use HRP Polymer to Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (AB250875)
  • WB

Lab

Western blot - Anti-Nkx3.1 antibody [EPR14970] - BSA and Azide free (AB250875)

This data was developed using ab186413, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Nkx3.1 antibody [EPR14970] (<a href='/en-us/products/primary-antibodies/nkx31-antibody-epr14970-ab186413'>ab186413</a>) at 0.988 µg/mL

Lane 1:

LNCaP cell lysate at 20 µg

Lane 2:

PC3 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 26 kDa

Observed band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14970

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

ab250875 is the carrier-free version of ab186413.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx3.1 known also as NK-3 transcription factor related locus 1 is a protein that acts as a transcription factor. Its molecular mass is approximately 25 kDa. The expression of Nkx3.1 occurs mostly in the prostate but you can also see it in other tissues like testis and certain brain regions. It plays roles in androgen receptor signaling where it binds to specific DNA sequences to regulate the transcription of target genes influencing cell growth and differentiation.
Biological function summary

Nkx3.1 is involved in regulating prostate epithelial cell differentiation and maintaining proper glandular architecture. It does not appear to form part of a larger protein complex but instead interacts directly with DNA to control gene expression. Alongside this it may involve co-regulatory proteins to execute its transcriptional activities effectively controlling the expression of genes critical for normal prostate function.

Pathways

Nkx3.1 is involved in the prostate cancer pathway and the androgen receptor signaling pathway. Within these pathways its interactions include those with proteins such as the androgen receptor (AR) and the homeobox protein Nkx2-5 coordinating the cellular responses to hormonal signals. These interactions allow Nkx3.1 to modulate the transcription of genes responsive to androgens which impacts cellular proliferation and development within prostate tissues.

Alterations in the Nkx3.1 protein function relate most notably to prostate cancer and benign prostatic hyperplasia (BPH). Loss or mutation of the Nkx3.1 gene often coincides with early stages of prostate cancer development. Through the androgen receptor pathway Nkx3.1 influences disease progression where decreased expression or activity can exacerbate cellular abnormalities. Understanding its altered interactions in these diseases particularly with the androgen receptor provides insight into mechanisms driving prostate disorders offering potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
See full target information NKX3-1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

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