Rabbit Recombinant Monoclonal Nkx3.1 antibody. Carrier free. Suitable for WB, IHC-P, mIHC and reacts with Human samples.
Images
![Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--western-blot-img108702.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49751.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217)")
![Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--western-blot-img58059.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135710.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217)")
![Multiplex immunohistochemistry - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--multiplex-immunohistochemistry-img408561.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (AB251217)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | mIHC | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.
Alternative names
NKX3.1, NKX3A, NKX3-1, Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A
Recommended products
Rabbit Recombinant Monoclonal Nkx3.1 antibody. Carrier free. Suitable for WB, IHC-P, mIHC and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16653
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251217 is the carrier-free version of Anti-Nkx3.1 antibody [EPR16653] ab196020.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nkx3.1 known also as NK-3 transcription factor related locus 1 is a protein that acts as a transcription factor. Its molecular mass is approximately 25 kDa. The expression of Nkx3.1 occurs mostly in the prostate but you can also see it in other tissues like testis and certain brain regions. It plays roles in androgen receptor signaling where it binds to specific DNA sequences to regulate the transcription of target genes influencing cell growth and differentiation.
- Biological function summary
Nkx3.1 is involved in regulating prostate epithelial cell differentiation and maintaining proper glandular architecture. It does not appear to form part of a larger protein complex but instead interacts directly with DNA to control gene expression. Alongside this it may involve co-regulatory proteins to execute its transcriptional activities effectively controlling the expression of genes critical for normal prostate function.
- Pathways
Nkx3.1 is involved in the prostate cancer pathway and the androgen receptor signaling pathway. Within these pathways its interactions include those with proteins such as the androgen receptor (AR) and the homeobox protein Nkx2-5 coordinating the cellular responses to hormonal signals. These interactions allow Nkx3.1 to modulate the transcription of genes responsive to androgens which impacts cellular proliferation and development within prostate tissues.
- Associated diseases and disorders
Alterations in the Nkx3.1 protein function relate most notably to prostate cancer and benign prostatic hyperplasia (BPH). Loss or mutation of the Nkx3.1 gene often coincides with early stages of prostate cancer development. Through the androgen receptor pathway Nkx3.1 influences disease progression where decreased expression or activity can exacerbate cellular abnormalities. Understanding its altered interactions in these diseases particularly with the androgen receptor provides insight into mechanisms driving prostate disorders offering potential targets for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--western-blot-img108702.jpg "Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)")
Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
This data was developed using Anti-Nkx3.1 antibody [EPR16653] ab196020, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Lane 2, PC-3 (Human prostate cancer cell line) is acting as a negative control for Nkx3.1. The 32kDa band may represent the glycosylated form.
All lanes: Western blot - Anti-Nkx3.1 antibody [EPR16653] (Anti-Nkx3.1 antibody [EPR16653] ab196020) at 1/1000 dilution
Lane 1: LNCaP (Human prostate cancer cell line) cell lysate at 10 µg
Lane 2: PC-3 (Human prostate cancer cell line) cell lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img49751.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
This data was developed using Anti-Nkx3.1 antibody [EPR16653] ab196020, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate hyperplasia tissue labeling Nkx3.1 with Anti-Nkx3.1 antibody [EPR16653] ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human prostate hyperplasia tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--western-blot-img58059.jpg "Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)")
Western blot - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
This data was developed using Anti-Nkx3.1 antibody [EPR16653] ab196020, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Nkx3.1 antibody [EPR16653] (Anti-Nkx3.1 antibody [EPR16653] ab196020) at 1/1000 dilution
All lanes: Human prostate lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 26 kDa
Observed band size: 32 kDa
Exposure time: 3min
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135710.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
This data was developed using Anti-Nkx3.1 antibody [EPR16653] ab196020, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling Nkx3.1 with Anti-Nkx3.1 antibody [EPR16653] ab196020 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) secondary antibody at 1/500 dilution. Human testis tissue acting as a negative control for Nkx3.1. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Multiplex immunohistochemistry - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217), expandable thumbnail](https://content.abcam.com/products/images/nkx31-antibody-epr16653-bsa-and-azide-free-ab251217--multiplex-immunohistochemistry-img408561.jpg "Multiplex immunohistochemistry - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)")
Multiplex immunohistochemistry - Anti-Nkx3.1 antibody [EPR16653] - BSA and Azide free (ab251217)
Fluorescence multiplex immunohistochemical analysis of human prostate gland tissue (formalin/PFA-fixed paraffin-embedded section). Panel A: merged staining of anti-Prostate Specific Antigen (Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113, red; Opal™690), anti-Cytokeratin 5 (Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free ab236216, green; Opal™520) and anti-Nkx3.1 (Anti-Nkx3.1 antibody [EPR16653] ab196020, gray; Opal™570) on human prostate gland tissue. Panel B: anti-Prostate Specific Antigen stained on cytoplasm of luminal cells. Panel C: anti-Cytokeratin 5 stained on basal cells. Panel D: anti-p63 stained on nucleus of luminal cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. The section was incubated in three rounds of staining: in the order of Anti-Prostate Specific Antigen antibody [EP1588Y] ab76113 (1/2000), Anti-Cytokeratin 5 antibody [SP27] - BSA and Azide free ab236216 (1/400) and Anti-Nkx3.1 antibody [EPR16653] ab196020 (1/2000) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) was used for 20 mins. DAPI (blue) was used as a nuclear counter stain. Image acquisition was performed with Leica SP8 confocal microscope.
This data was developed using Anti-Nkx3.1 antibody [EPR16653] ab196020, the same antibody clone in a different buffer formulation.
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