AB257325

Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free

Name: Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (ab257325) | Abcam
Brand: Abcam Limited
SKU: AB257325
Price: 905 USD
Availability: InStock

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Mouse Monoclonal Nkx3.1 antibody. Carrier free. Suitable for IHC-P, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human NKX3-1 aa 50 to C-terminus.

View Alternative Names

NKX3.1, NKX3A, NKX3-1, Homeobox protein Nkx-3.1, Homeobox protein NK-3 homolog A

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (AB257325)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (AB257325)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Nkx3.1 using ab257308 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was produced with ab257308, the same antibody in a different formulation with BSA and Azide.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (AB257325)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling Nkx3.1 using ab257308 at 2 μg/ml in immunohistochemical analysis.

Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

This data was produced with ab257308, the same antibody in a different formulation with BSA and Azide.

Protein Array - Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (AB257325)

Protein Array

Supplier Data

Protein Array - Anti-Nkx3.1 antibody [NKX3.1/2576] - BSA and Azide free (AB257325)

Analysis of >19,000 full-length human proteins using ab257308.

This data was produced with ab257308, the same antibody in a different formulation with BSA and Azide.

Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NKX3.1/2576

Isotype

IgG2c

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human NKX3-1 aa 50 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99801

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "" }, "Recombinant full length protein - Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>" } } }

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Product details

ab257325 is the carrier-free version of ab257308.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from bioreactor concentrate.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Nkx3.1 known also as NK-3 transcription factor related locus 1 is a protein that acts as a transcription factor. Its molecular mass is approximately 25 kDa. The expression of Nkx3.1 occurs mostly in the prostate but you can also see it in other tissues like testis and certain brain regions. It plays roles in androgen receptor signaling where it binds to specific DNA sequences to regulate the transcription of target genes influencing cell growth and differentiation.

Biological function summary

Nkx3.1 is involved in regulating prostate epithelial cell differentiation and maintaining proper glandular architecture. It does not appear to form part of a larger protein complex but instead interacts directly with DNA to control gene expression. Alongside this it may involve co-regulatory proteins to execute its transcriptional activities effectively controlling the expression of genes critical for normal prostate function.

Pathways

Nkx3.1 is involved in the prostate cancer pathway and the androgen receptor signaling pathway. Within these pathways its interactions include those with proteins such as the androgen receptor (AR) and the homeobox protein Nkx2-5 coordinating the cellular responses to hormonal signals. These interactions allow Nkx3.1 to modulate the transcription of genes responsive to androgens which impacts cellular proliferation and development within prostate tissues.

Alterations in the Nkx3.1 protein function relate most notably to prostate cancer and benign prostatic hyperplasia (BPH). Loss or mutation of the Nkx3.1 gene often coincides with early stages of prostate cancer development. Through the androgen receptor pathway Nkx3.1 influences disease progression where decreased expression or activity can exacerbate cellular abnormalities. Understanding its altered interactions in these diseases particularly with the androgen receptor provides insight into mechanisms driving prostate disorders offering potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor, which binds preferentially the consensus sequence 5'-TAAGT[AG]-3' and can behave as a transcriptional repressor. Plays an important role in normal prostate development, regulating proliferation of glandular epithelium and in the formation of ducts in prostate. Acts as a tumor suppressor controlling prostate carcinogenesis, as shown by the ability to inhibit proliferation and invasion activities of PC-3 prostate cancer cells.

See full target information NKX3-1

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