Name: Anti-nkx6.1 antibody [EPR20405]
SKU: ab221549
Rating: 5 (2 reviews)

Anti-nkx6.1 antibody [EPR20405] (ab221549) is a rabbit monoclonal antibody detecting nkx6.1 in Western Blot, IHC-P, IHC-Fr. Suitable for Human, Mouse.

- Biophysical QC for unrivalled batch-batch consistency

- Over 10 publications

Images

Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (AB221549), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-Fr	IHC-P
Human	Tested	Expected	Tested
Mouse	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

See full target information NKX6-1

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).

Alternative names

NKX6A, NKX6-1, Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20405
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-nkx6.1 antibody [EPR20405] (ab221549) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse samples.

What is the molecular weight of nkx6.1?

Anti-nkx6.1 [EPR20405] (ab221549) specifically detects a band for nkx6.1 (UniProt: P78426) at a molecular weight of 38kDa.

Trusted by the scientific community

Anti-nkx6.1 [EPR20405] (ab221549) was first used in a scientific publication in 2017 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR20405] also available for your convenience:
ab221549, Carrier free - ab240398, Alexa Fluor® 647 - ab322233

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Nkx6.1 is a homeodomain-containing transcription factor also known as NKX6A. It weighs approximately 36 kDa and is mainly expressed in the developing central nervous system and pancreas. In the pancreas it is found during early developmental stages in islet and exocrine cells. The protein acts as a transcriptional regulator and influences the expression of genes important for cell differentiation and development.

Biological function summary

Nkx6.1 plays a significant role in pancreatic beta-cell lineage development. It regulates genes that are essential for maintaining beta-cell identity and functions such as insulin gene transcription. Nkx6.1 functions as a part of a transcription factor complex and works in conjunction with other proteins to achieve its effects. It upregulates target genes necessary for cell cycle progression and insulin regulation ensuring proper beta-cell function.

Pathways

Nkx6.1 is strongly associated with the pancreatic development pathway and cell differentiation processes. It works closely with proteins like Pax4 and Pax6 to control the fate of pancreatic progenitor cells. The Notch signaling pathway also involves Nkx6.1 where it influences the determination of specific cell types in the pancreas. This interaction helps regulate the balance between proliferation and differentiation of precursor cells.

Associated diseases and disorders

Nkx6.1 has connections to diabetes especially type 1 diabetes as it affects insulin-producing beta-cells. A deficiency or malfunction in Nkx6.1 can lead to impaired insulin synthesis and secretion. Nkx2.2 is another protein involved in the pancreatic beta-cell function and shares regulatory pathways with Nkx6.1. Additionally research hints at Nkx6.1 involvement in certain central nervous system disorders since alterations in its expression could result in developmental abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling nkx6.1 with ab221549 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on islet of mouse pancreas is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: no staining on human colon (PMID: 25871618).
Counter stained with hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Blocking/Dilution buffer: 5% NFDM/TBST.
Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions.
All lanes: Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution
All lanes: Human fetal pancreas lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 3min
Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549)
Blocking/Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-nkx6.1 antibody [EPR20405] (ab221549) at 1/1000 dilution
All lanes: Beta-TC-6 (mouse pancreas insulinoma beta cell) whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 30s

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Anti-nkx6.1 antibody [EPR20405]

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