Rabbit Recombinant Monoclonal nkx6.1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Human samples.
Images
![Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-frozen-sections-img120296.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82256.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100172.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91247.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44820.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (AB240398)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-Fr | IHC-P | |
|---|---|---|---|
| Human | Expected | Expected | Tested |
| Mouse | Expected | Tested | Expected |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Human | Dilution info - | Notes Under our testing conditions we used a high sensitivity exposure system to obtain this result. Customers are recommended to optimize WB testing conditions. |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).
Alternative names
NKX6A, NKX6-1, Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A
Recommended products
Rabbit Recombinant Monoclonal nkx6.1 antibody. Carrier free. Suitable for WB, IHC-Fr, IHC-P and reacts with Mouse, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20405
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240398 is the carrier-free version of Anti-nkx6.1 antibody [EPR20405] ab221549.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Nkx6.1 is a homeodomain-containing transcription factor also known as NKX6A. It weighs approximately 36 kDa and is mainly expressed in the developing central nervous system and pancreas. In the pancreas it is found during early developmental stages in islet and exocrine cells. The protein acts as a transcriptional regulator and influences the expression of genes important for cell differentiation and development.
- Biological function summary
Nkx6.1 plays a significant role in pancreatic beta-cell lineage development. It regulates genes that are essential for maintaining beta-cell identity and functions such as insulin gene transcription. Nkx6.1 functions as a part of a transcription factor complex and works in conjunction with other proteins to achieve its effects. It upregulates target genes necessary for cell cycle progression and insulin regulation ensuring proper beta-cell function.
- Pathways
Nkx6.1 is strongly associated with the pancreatic development pathway and cell differentiation processes. It works closely with proteins like Pax4 and Pax6 to control the fate of pancreatic progenitor cells. The Notch signaling pathway also involves Nkx6.1 where it influences the determination of specific cell types in the pancreas. This interaction helps regulate the balance between proliferation and differentiation of precursor cells.
- Associated diseases and disorders
Nkx6.1 has connections to diabetes especially type 1 diabetes as it affects insulin-producing beta-cells. A deficiency or malfunction in Nkx6.1 can lead to impaired insulin synthesis and secretion. Nkx2.2 is another protein involved in the pancreatic beta-cell function and shares regulatory pathways with Nkx6.1. Additionally research hints at Nkx6.1 involvement in certain central nervous system disorders since alterations in its expression could result in developmental abnormalities.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-frozen-sections-img120296.jpg "Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)")
Immunohistochemistry (Frozen sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling nkx6.1 with Anti-nkx6.1 antibody [EPR20405] ab221549 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining on islet of mouse pancreas is observed.
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-nkx6.1 antibody [EPR20405] ab221549).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82256.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling nkx6.1 with Anti-nkx6.1 antibody [EPR20405] ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of human pancreas (PMID: 8702531). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-nkx6.1 antibody [EPR20405] ab221549).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100172.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
Immunohistochemical analysis of paraffin-embedded human pancreatic neuroendocrine neoplasm tissue labeling nkx6.1 with Anti-nkx6.1 antibody [EPR20405] ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Mainly nuclear staining on tumor cells of human pancreatic neuroendocrine neoplasm (PMID: 25871618). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-nkx6.1 antibody [EPR20405] ab221549).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img91247.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling nkx6.1 with Anti-nkx6.1 antibody [EPR20405] ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on islet of mouse pancreas (PMID: 8702531). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-nkx6.1 antibody [EPR20405] ab221549).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398), expandable thumbnail](https://content.abcam.com/products/images/nkx61-antibody-epr20405-bsa-and-azide-free-ab240398--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44820.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [EPR20405] - BSA and Azide free (ab240398)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling nkx6.1 with Anti-nkx6.1 antibody [EPR20405] ab221549 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative control: no staining on human colon (PMID: 25871618).
Counter stained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-nkx6.1 antibody [EPR20405] ab221549).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com