Skip to main content

Mouse Monoclonal nkx6.1 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NKX6-1.

Be the first to review this product! Submit a review

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (AB268088), expandable thumbnail
  • Protein Array - Anti-nkx6.1 antibody [NKX61/2561] (AB268088), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (AB268088), expandable thumbnail

Key facts

Isotype
IgG2c
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human NKX6-1. Database link P78426

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Protein ArrayIHC-P
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1.00000-2.00000 µg/mL
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

1 product for Alternative Version

Target data

Function

Transcription factor which binds to specific A/T-rich DNA sequences in the promoter regions of a number of genes. Involved in the development of insulin-producing beta cells in the islets of Langerhans at the secondary transition (By similarity). Together with NKX2-2 and IRX3 acts to restrict the generation of motor neurons to the appropriate region of the neural tube. Belongs to the class II proteins of neuronal progenitor factors, which are induced by SHH signals (By similarity).

Alternative names

NKX6A, NKX6-1, Homeobox protein Nkx-6.1, Homeobox protein NK-6 homolog A

Recommended products

Mouse Monoclonal nkx6.1 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NKX6-1.

Key facts

Isotype
IgG2c
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human NKX6-1. Database link P78426
Clone number
NKX61/2561
Purification technique
Affinity purification Protein A/G
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from Bioreactor concentrate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Nkx6.1 is a homeodomain-containing transcription factor also known as NKX6A. It weighs approximately 36 kDa and is mainly expressed in the developing central nervous system and pancreas. In the pancreas it is found during early developmental stages in islet and exocrine cells. The protein acts as a transcriptional regulator and influences the expression of genes important for cell differentiation and development.

Biological function summary

Nkx6.1 plays a significant role in pancreatic beta-cell lineage development. It regulates genes that are essential for maintaining beta-cell identity and functions such as insulin gene transcription. Nkx6.1 functions as a part of a transcription factor complex and works in conjunction with other proteins to achieve its effects. It upregulates target genes necessary for cell cycle progression and insulin regulation ensuring proper beta-cell function.

Pathways

Nkx6.1 is strongly associated with the pancreatic development pathway and cell differentiation processes. It works closely with proteins like Pax4 and Pax6 to control the fate of pancreatic progenitor cells. The Notch signaling pathway also involves Nkx6.1 where it influences the determination of specific cell types in the pancreas. This interaction helps regulate the balance between proliferation and differentiation of precursor cells.

Associated diseases and disorders

Nkx6.1 has connections to diabetes especially type 1 diabetes as it affects insulin-producing beta-cells. A deficiency or malfunction in Nkx6.1 can lead to impaired insulin synthesis and secretion. Nkx2.2 is another protein involved in the pancreatic beta-cell function and shares regulatory pathways with Nkx6.1. Additionally research hints at Nkx6.1 involvement in certain central nervous system disorders since alterations in its expression could result in developmental abnormalities.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (ab268088), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (ab268088)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for nkx6.1 with ab268088 at 2 μg/ml in immunohistochemical analysis.

  • Protein Array - Anti-nkx6.1 antibody [NKX61/2561] (ab268088), expandable thumbnail

    Protein Array - Anti-nkx6.1 antibody [NKX61/2561] (ab268088)

    Protein Array containing more than 19,000 full-length human proteins using ab268088. Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (ab268088), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nkx6.1 antibody [NKX61/2561] (ab268088)

    Formalin-fixed, paraffin-embedded human pancreas tissue stained for nkx6.1 with ab268088 at 2 μg/ml in immunohistochemical analysis.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com